caa a22 4500 397494238 CHVBK 20180308164516.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jb/117.2.420 doi (NATIONALLICENCE)oxford-10.1093/jb/117.2.420 Pancreatic-Type Phospholipase A2 Activates Prostaglandin E2 Production in Rat Mesangial Cells by Receptor Binding Reaction [Elektronische Daten] [Junji Kishino, Keiko Kawamoto, Jun Ishizaki, Hubertus M. Verheij, Osamu Ohara, Hitoshi Arita] Our earlier studies have shown that mammalian pancreatic group I phospholipase A2 (PLA2-I) has its specific receptor (PLA2 receptor) on a wide range of mammalian cells and that the receptor-binding capability of PLA2 is a property of this molecule separable from its enzymatic activity. To clarify whether PLA2 activity is required for eliciting a biological response via the receptor or not, we examined the enzymatic activity of PLA2-I/PLA2 receptor complex and the inducibility of prostaglandin (PG) E2 production in rat mesangial cells by mutant PLA2S-I. Using a recombinant soluble PLA2 receptor, we first found that PLA2-I could not hydrolyze a phospholipid substrate when complexed with the receptor. In the next experiment using various mutant porcine PLA2S-I we found that PGE2 production in rat mesangial cells could be induced by a mutant PLA2-I which retained the receptor- binding activity but had almost completely lost its enzymatic activity. These findings indicate that the enzyme action of PLA2-I is not required for a PLA2-I-induced biological response, i.e., the augmentation of PGE2 production in rat mesangial cells. © BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence in vitro mutagenesis nationallicence pancreatic type I phospholipase A nationallicence prostaglandin nationallicence receptor binding nationallicence Kishino Junji Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut Kawamoto Keiko Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut Ishizaki Jun Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut Verheij Hubertus M. tDepartment of Enzymology and Protein Engineering, University of Utrecht CBLE, Utrecht, The Netherlands aut Ohara Osamu Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut Arita Hitoshi Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut The Journal of Biochemistry Oxford University Press 117/2(1995-02), 420-424 0021-924X 117:2<420 1995 117 jbchem https://doi.org/10.1093/jb/117.2.420 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/117.2.420 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kishino Junji Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut NATIONALLICENCE 700 1- Kawamoto Keiko Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut NATIONALLICENCE 700 1- Ishizaki Jun Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut NATIONALLICENCE 700 1- Verheij Hubertus M. tDepartment of Enzymology and Protein Engineering, University of Utrecht CBLE, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Ohara Osamu Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut NATIONALLICENCE 700 1- Arita Hitoshi Shionogi Research Laboratories, Shionogi & Company, Ltd., 12-4 Sagisu 5-chome, Fukushima-ku, Osaka 553 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/2(1995-02), 420-424 0021-924X 117:2<420 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford