caa a22 4500 397494254 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124961 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124961 Komiyama Makoto Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 Sequence-Specific and Hydrolytic Scission of DNA and RNA by Lanthanide Complex-OligoDNA Hybrids [Elektronische Daten] [Makoto Komiyama] Totally synthetic and sequence-specific nucleases and ribonucleases, which hydrolyze DNAs and RNAs selectively at target sites, have been prepared. Lanthanide ions, which efficiently hydrolyze the phosphodiester linkages in nucleic acids, are attached to the 5′-end of synthetic DNA oligomers (sequence-recognizing sites) by use of an iminodiacetate ligand. Under physiological conditions, the hybrids selectively hydrolyze substrate DNA or RNA at the 3′-side of the sequence which is complementary with the DNA oligomers in the hybrids. The cerium(IV) ion is the most active as to DNA scission, whereas the europium(III), thulium(III), and lutetium(III) ions are the most effective for RNA scission. All the scissions proceed totally via hydrolysis of the phosphodiester linkages, in the same way as the scissions by natural nucleases and ribonucleases do. The artificial enzymes, which show far greater sequence-specificities than natural ones, exhibit high potential for application to molecular biology, biotechnology, and therapy. © Oxford University Press JB Reviews nationallicence cerium ion nationallicence DNA hydrolysis nationallicence lanthanide complex nationallicence lutetium ion nationallicence RNA hydrolysis nationallicence The Journal of Biochemistry Oxford University Press 118/4(1995-10), 665-670 0021-924X 118:4<665 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124961 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124961 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Komiyama Makoto Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/4(1995-10), 665-670 0021-924X 118:4<665 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford