caa a22 4500 397494262 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jb/117.2.346 doi (NATIONALLICENCE)oxford-10.1093/jb/117.2.346 Human Gene for β-Microseminoprotein: Its Promoter Structure and Chromosomal Localization [Elektronische Daten] [Yukiko Ochiai, Johji Inazawa, Hisao Ueyama, Iwao Ohkubo] The gene for human β-microseminoprotein (MSP, β-inhibin, prostatic secretory protein94 or PSP94 was isolated. The nucleotide sequence of its upstream region (2,860 bp), exon 1(35 bp), and a part of the following intron (218 bp) was determined. Transient transfection analysis on MSP-expressing (PC-3) and -nonexpressing (HepG2) cells using luciferase reporter plasmids suggested that the region from -2738 to -276 is not essential for the basal promoter activity, and that the regions from -275 to -207 and from -186 to -128 function in a cell-specific manner. The chromosome locus of the gene (MSMB) was determined through application of PCR to the DNAs of rodent-human somatic cell hybrids and also by the fluorescence in situ hybridization technique to be region qll.2 of chromo some 10. © BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence chromosomal localization nationallicence gene structure nationallicence human seminal plasma nationallicence β-micro seminoprotein nationallicence transient expression assay nationallicence Ochiai Yukiko Department of Medical Biochemistry, Shiga University of Medical Science Seta, Otsu, Shiga 520-21 aut Inazawa Johji Department of Hygiene, Kyoto Prefectural University of Medicine Kamigyo-ku, Kyoto 602 aut Ueyama Hisao Department of Medical Biochemistry, Shiga University of Medical Science Seta, Otsu, Shiga 520-21 aut Ohkubo Iwao Department of Medical Biochemistry, Shiga University of Medical Science Seta, Otsu, Shiga 520-21 aut The Journal of Biochemistry Oxford University Press 117/2(1995-02), 346-352 0021-924X 117:2<346 1995 117 jbchem https://doi.org/10.1093/jb/117.2.346 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/117.2.346 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ochiai Yukiko Department of Medical Biochemistry, Shiga University of Medical Science Seta, Otsu, Shiga 520-21 aut NATIONALLICENCE 700 1- Inazawa Johji Department of Hygiene, Kyoto Prefectural University of Medicine Kamigyo-ku, Kyoto 602 aut NATIONALLICENCE 700 1- Ueyama Hisao Department of Medical Biochemistry, Shiga University of Medical Science Seta, Otsu, Shiga 520-21 aut NATIONALLICENCE 700 1- Ohkubo Iwao Department of Medical Biochemistry, Shiga University of Medical Science Seta, Otsu, Shiga 520-21 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/2(1995-02), 346-352 0021-924X 117:2<346 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford