caa a22 4500 397494319 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/jb/118.5.1001 doi (NATIONALLICENCE)oxford-10.1093/jb/118.5.1001 Inhibitory Effect of a Synthetic Protease Inhibitor (Gabexate Mesilate on the Respiratory Burst Oxidase in Human Neutrophils [Elektronische Daten] [Ichiro Ohashi, Junichi Nishijima, Atsuo Murata, Hitoshi Toda, Takeshi Kato, Hirohito Hayashida, Hitoshi Shiozaki, Morito Monden] We investigated the mechanism by which the synthetic proetease inhibitor gabexate mesilate inhibits the production of the superoxide anion by human neutrophils. We found that gabexate mesilate suppressed SOD-inhibitable cytochrome c reduction in a dose-dependent manner in intact neutrophils activated with phorbol ester. Gabexate mesilate slightly scavenged the superoxide anion in the pyrogallol assay. The reagent also inhibited superoxide anion production in a dose-dependent manner in a cell-free oxidase-activating system. Translocation of the cytosolic respiratory burst oxidase components, the 47- and 65-kDa proteins, to membranes was suppressed by the reagent in intact cells stimulated with phorbol ester. Gabexate mesilate also reduced arachidonic acid-induced translocation of the components to the membrane fraction in the cell-free system. These results demonstrate that gabexate mesilate suppresses superoxide anion production by reducing the translocation of the 47- and 65-kDa proteins to the plasma membrane. © Oxford University Press Regular Papers nationallicence gabexate mesilate nationallicence neutrophil nationallicence respiratory burst oxidase nationallicence superoxide nationallicence Ohashi Ichiro Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Nishijima Junichi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Murata Atsuo Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Toda Hitoshi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Kato Takeshi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Hayashida Hirohito Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Shiozaki Hitoshi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut Monden Morito Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 118/5(1995-10), 1001-1006 0021-924X 118:5<1001 1995 118 jbchem https://doi.org/10.1093/jb/118.5.1001 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/118.5.1001 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohashi Ichiro Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Nishijima Junichi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Murata Atsuo Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Toda Hitoshi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Kato Takeshi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Hayashida Hirohito Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Shiozaki Hitoshi Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Monden Morito Department of Surgery II, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/5(1995-10), 1001-1006 0021-924X 118:5<1001 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford