caa a22 4500 397494351 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jb/117.2.315 doi (NATIONALLICENCE)oxford-10.1093/jb/117.2.315 Interleukin 4 Enhances Ganglioside GD3 Expression on the Human Fibroblast Cell Line WI-38 [Elektronische Daten] [Byung Ook Choi, Toshiaki Yamaki, Yukihiro Ibayashi, Yoshihiro Maeda, Shinsei Gasa, Kazuo Hashi] Human fibroblast cell line WI-38 cultured in vitro was treated with a human recombinant IL-4 at concentrations of 1 to 100 U/mi to examine the alteration of glycosphingolipid (GSL) expression of the cells. Neutral GSL of non-treated WI-38 cells consisted of CMH (GicCer), CDH, CTH, and Gb CMH and CTH were the major components. The acidic GSL were composed of GM3 as the predominant component and other minor gangliosides including GD3. The neutral GSLs did not change in profile during the treatment with IL-4, while the acidic GSLs showed a prominent change, an increase of GD3 content. The increase of GD3 was detectable with IL-4 concentrations over 1 U/ml, and reached a plateau at 10 U/ml, where the amount of GD3 was almost equal to that of GM3. The GD3 increase occurred at 24 h after the IL-4 treatment, and lasted for at least 96 h, as long as IL-4 remained present in the culture media. The GD3 synthase (sialyltransferase) level was found to be increased in an IL-4 dose-dependent manner. IL-4 did not influence the growth or morphological appearance of WI-38 cells. The results demonstrate a novel biological effect of IL-4, modulating GSL in non-hematopoietic cells. © BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence fibroblast nationallicence ganglioside nationallicence GD3 nationallicence interleukin 4 nationallicence sialyltransferase nationallicence Choi Byung Ook Department of Chemistry, Sapporo Medical University, School of Medicine, S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060, S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut Yamaki Toshiaki Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut Ibayashi Yukihiro Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut Maeda Yoshihiro Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut Gasa Shinsei Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut Hashi Kazuo Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut The Journal of Biochemistry Oxford University Press 117/2(1995-02), 315-320 0021-924X 117:2<315 1995 117 jbchem https://doi.org/10.1093/jb/117.2.315 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/117.2.315 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Choi Byung Ook Department of Chemistry, Sapporo Medical University, School of Medicine, S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060, S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Yamaki Toshiaki Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Ibayashi Yukihiro Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Maeda Yoshihiro Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Gasa Shinsei Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Hashi Kazuo Department of Chemistry, Sapporo Medical University, School of Medicine S-i, W-16, Chuo-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/2(1995-02), 315-320 0021-924X 117:2<315 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford