caa a22 4500 39749436X CHVBK 20180308164517.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jb/117.2.237 doi (NATIONALLICENCE)oxford-10.1093/jb/117.2.237 Detection of NG,NGDimethylarginine Dimethylaminohydrolase in Human Tissues Using a Monoclonal Antibody [Elektronische Daten] [Masumi Kimoto, Guy St. J. Whitley, Hideaki Tsuji, Tadashi Ogawa] Two endogenous inhibitors of nitric oxide synthesis, NG-monomethylarginine (MMA) and NG,NG-dimethylarginine (DMA), are broken down by NG,NG-dimethylarginine dimethylaminohydrolase (DDAH) EEC 3.5.3.18]. This enzymatic activity is widely distributed in rat tissues, and is correlated with the distribution of free MMA and DMA, and the production of nitric oxide. In this study, immunoblotting analyses with a monoclonal antibody directed towards rat kidney DDAH showed that immunoreactive proteins exist in human pancreas, kidney, and liver. Enzyme activity was definitely detected in these tissues, but the specific activity was lower than that in the corresponding rat tissue. These results are interesting in connection with the idea that the enzyme might play a role as a regulator of nitric oxide generation in human tissues. © BY THE JOURNAL OF BIOCHEMISTRY Rapid Communication nationallicence NG ,NG-dimethylarginine dimethylaminohydrolase nationallicence nitric oxide generation nationallicence human tissues nationallicence Kimoto Masumi Department of Nutrition, School of Medicine, The University of Tokushima, Kuramoto-cho Tokushima, Tokushima 770 aut Whitley Guy St. J. Department of Cellular and Molecular Sciences, St George's Hospital Medical School Cranmer Terrace, London SW17 ORE, UK aut Tsuji Hideaki Department of Nutrition, School of Medicine, The University of Tokushima, Kuramoto-cho Tokushima, Tokushima 770 aut Ogawa Tadashi Department of Nutrition, School of Medicine, The University of Tokushima, Kuramoto-cho Tokushima, Tokushima 770 aut The Journal of Biochemistry Oxford University Press 117/2(1995-02), 237-238 0021-924X 117:2<237 1995 117 jbchem https://doi.org/10.1093/jb/117.2.237 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jb/117.2.237 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kimoto Masumi Department of Nutrition, School of Medicine, The University of Tokushima, Kuramoto-cho Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Whitley Guy St. J. Department of Cellular and Molecular Sciences, St George's Hospital Medical School Cranmer Terrace, London SW17 ORE, UK aut NATIONALLICENCE 700 1- Tsuji Hideaki Department of Nutrition, School of Medicine, The University of Tokushima, Kuramoto-cho Tokushima, Tokushima 770 aut NATIONALLICENCE 700 1- Ogawa Tadashi Department of Nutrition, School of Medicine, The University of Tokushima, Kuramoto-cho Tokushima, Tokushima 770 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/2(1995-02), 237-238 0021-924X 117:2<237 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford