caa a22 4500 397494424 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199508 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124928 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124928 Expression of Calcitonin Receptors on Human Myeloid Leukemia Cells [Elektronische Daten] [Kinue Suzuki, Masako Uchii, Ryushi Nozawa] Certain osteoclastic markers (multinueleation and tartrate-resistant acid phosphatase) were induced in human leukemia HL-60 cells by treatment with 107 M 1, 25-dihydroxy-vitamin D3 (1, 25(OH)2D3) for 10 days. However, no formation of pits on a bone substrate by vitamin-treated HL-60 cells was detected. Expression of calcitonin receptors (CTR), another osteoclastic marker, was examined by means of the reverse transcriptase polymerase chain reaction. The human CTR-cDNA (T47D isotype) was amplified from untreated HL-60 cells, but not from cells treated with 1,25(OH)2D3. The CTR mRNA disappeared within 24 h after the treatment. Thus, 1, 25(OH)2D3-differentiated HL-60 cells failed to show two intrinsic characteristics of osteoclasts, pit formation on a bone substrate and expression of CTR. We then examined the expression of CTR on established human leukemia cell lines. The CTR mRNA was expressed in myeloblastic ML-1 and promyelocytic HL-60 leukemia cells but not in more mature macrophage-like cell lines, U-937 and THP-1 cells. Neither B cell leukemia BALL-1, T cell leukemia Jurkat, promegakaryoblastic leukemia Meg-J, nor cervix uteri carcinoma HeLa S3 cells amplified the CTR products. The cDNA of BIN67-isotype CTR, that has an additional 16-amino acid insert in the putative first intracellular loop of T47D-type CTR (Kuestner et al.(1994) MoL Pharmacol. 46, 246-255), was amplified by neither strain tested. It was suggested that the T47D-type CTR is a novel differentiation antigen of immature myeloid lineage cells. © by the Journal of Biochemistry Regular Papers nationallicence calcitonin receptors nationallicence differentiation antigen nationallicence 1, 25-thydroxyvitamin D3 nationallicence lcukemia cells nationallicence osteoclasts nationallicence Suzuki Kinue Laboratory of Microbiology and Host Defenses, University of Shizuoka School of Food and Nutritional Sciences, 52-1 yata, Shizuoka, Shizuoka 422 aut Uchii Masako Laboratory of Microbiology and Host Defenses, University of Shizuoka School of Food and Nutritional Sciences, 52-1 yata, Shizuoka, Shizuoka 422 aut Nozawa Ryushi Laboratory of Microbiology and Host Defenses, University of Shizuoka School of Food and Nutritional Sciences, 52-1 yata, Shizuoka, Shizuoka 422 aut The Journal of Biochemistry Oxford University Press 118/2(1995-08), 448-452 0021-924X 118:2<448 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124928 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124928 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suzuki Kinue Laboratory of Microbiology and Host Defenses, University of Shizuoka School of Food and Nutritional Sciences, 52-1 yata, Shizuoka, Shizuoka 422 aut NATIONALLICENCE 700 1- Uchii Masako Laboratory of Microbiology and Host Defenses, University of Shizuoka School of Food and Nutritional Sciences, 52-1 yata, Shizuoka, Shizuoka 422 aut NATIONALLICENCE 700 1- Nozawa Ryushi Laboratory of Microbiology and Host Defenses, University of Shizuoka School of Food and Nutritional Sciences, 52-1 yata, Shizuoka, Shizuoka 422 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/2(1995-08), 448-452 0021-924X 118:2<448 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford