caa a22 4500 397494432 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199507 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124883 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124883 Purification and Characterization of Thrombopoietin [Elektronische Daten] [Takashi Kato, Kinya Ogami, Yoshihiro Shimada, Akihiro Iwamatsu, Yoshiaki Sohma, Hiromichi Akahori, Kaori Horie, Atsuko Kokubo, Yoko Kudo, Emiko Maeda, Keiko Kobayashi, Hideya Ohashi, Tadashi Ozawa, Hideo Inoue, Kazuo Kawamura, Hiroshi Miyazaki] A thrombopoietic factor, termed thrombopoietin (TPO), was highly purified directly from the plasma of sublethally irradiated 1, 100 rats by measuring the production of megakaryo-cytes from a highly enriched population of rat megakaryocyte progenitor cells (CFU-MK). The rat plasma TPO is a glycoprotein and strongly hydrophobic. The total activity and purification yields obtained were about 29% and 1.49%108, respectively. The amino acid sequences of the two peptide fragments prepared from the purified 19 kDa TPO were analyzed, and used for the cloning of rat and human TPO cDNAs. It was found that the 19 kDa TPO was truncated but comprised at least 163 amino acids. The sequence of human TPO cDNA revealed that the TPO was identical to the c-Mpl ligand. Both rat and human TPOs expressed in COS-1 cells exhibited significant activity toward the CFU-MK in vitro and were active in stimulating platelet production in mice. These results indicate that a thrombopoietic factor originally found in the irradiated rat plasma is a ligand for the rat c-Mpl. © by the Journal of Biochemistry Regular Papers nationallicence Key words nationallicence c-Mpl ligand nationallicence megakaryocyte nationallicence plasma protein purification nationallicence platelet nationallicence throm-bopoietin nationallicence Kato Takashi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Ogami Kinya Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Shimada Yoshihiro Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Iwamatsu Akihiro Central Laboratories for Key Technology Kirin Brewery Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236 aut Sohma Yoshiaki Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Akahori Hiromichi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Horie Kaori Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Kokubo Atsuko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Kudo Yoko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Maeda Emiko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Kobayashi Keiko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Ohashi Hideya Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Ozawa Tadashi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Inoue Hideo Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Kawamura Kazuo Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut Miyazaki Hiroshi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut The Journal of Biochemistry Oxford University Press 118/1(1995-07), 229-236 0021-924X 118:1<229 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124883 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124883 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato Takashi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Ogami Kinya Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Shimada Yoshihiro Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Iwamatsu Akihiro Central Laboratories for Key Technology Kirin Brewery Co., Ltd., 1-13-5 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236 aut NATIONALLICENCE 700 1- Sohma Yoshiaki Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Akahori Hiromichi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Horie Kaori Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Kokubo Atsuko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Kudo Yoko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Maeda Emiko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Kobayashi Keiko Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Ohashi Hideya Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Ozawa Tadashi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Inoue Hideo Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Kawamura Kazuo Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Miyazaki Hiroshi Pharmaceutical Research Laboratory Kirin Brewery Co., Ltd., 1-2-2 Soujamachi, Maebashi, Gunma 371 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/1(1995-07), 229-236 0021-924X 118:1<229 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford