caa a22 4500 397494459 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124845 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124845 Possible Involvement of Protein-Tyrosine Kinases Such as p72syk in the Disc-Sphere Change Response of Porcine Platelets [Elektronische Daten] [Hiroyuki Maeda, Tetsuya Inazu, Katsuya Nagai, Shingo Maruyama, Gizou Nakagawara, Hirohei Yamamura] We previously reported that p72syk, a non-receptor tyrosine kinase, was activated maximally at 10 s after thrombin or thromboxane A2 stimulation, even in platelets that were not allowed to aggregate [Taniguchi et al. (1993) J. Biol. Chem. 268, 2277-2279; Maeda et al. (1993) Biochem. Biophys. Res. Commun. 197, 62-67]. Then, the change in the shape of porcine platelets induced by the thromboxane A2 analogue, STA2, and the role of protein-tyrosine kinases including p72syk in this response were evaluated, using the shape-change parameter. We show that p72syk activation is correlated with the disc-sphere change in a time- and dose-dependent manner following stimulation by STA2. Tyrphostin B44, a potent protein-tyrosine kinase inhibitor, reduced the thromboxane A2-evoked p72syk activation and the disc-sphere change in a dose-dependent manner. Furthermore, the translocation of p72syk to the cytoskeleton-rich fraction and an increase in the tyrosine phosphorylation of an about 120 kDa protein were observed during the disc-sphere change induced by STA2. These lines of evidence suggest that the activation of protein-tyrosine kinases such as p72syk may be involved in the disc-sphere change response in thromboxane A2-stimulated porcine platelets. © Oxford University Press Regular Papers nationallicence platelets nationallicence protein-tyrosine kinase nationallicence p72syk nationallicence shape change nationallicence thromboxane A2 nationallicence Maeda Hiroyuki Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut Inazu Tetsuya Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut Nagai Katsuya Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut Maruyama Shingo Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut Nakagawara Gizou Department of Surgery, Fukui Medical School Matsuoka, Fukui 910-11 aut Yamamura Hirohei Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1201-1208 0021-924X 117:6<1201 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124845 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124845 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maeda Hiroyuki Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Inazu Tetsuya Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Nagai Katsuya Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Maruyama Shingo Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Nakagawara Gizou Department of Surgery, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 700 1- Yamamura Hirohei Department of Biochemistry, Fukui Medical School Matsuoka, Fukui 910-11 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1201-1208 0021-924X 117:6<1201 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford