caa a22 4500 397494475 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124860 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124860 Mode of Action of an Antibacterial Peptide, KLKLLLLLKLK-NH2 [Elektronische Daten] [Juan Alvarez-Bravo, Shoichiro Kurata, Shunji Natori] Previously, we reported that a synthetic undecapeptide, KLKLLLLLKLK-NH2, and its d-enantiomer have potent bactericidal activities against both Gram-positive and Gram-negative bacteria. Here we examined the mode of action of KLKLLLLLKLK-NH2 with special reference to its effect on bacterial membranes. We found that both the outer and inner membrane of Escherichia coli become permeable to low molecular mass substances when treated with this peptide. Under these conditions, the bacteria lost the ability to synthesize ATP and to transport proline, suggesting that their electrochemical membrane potential was disrupted. This peptide appears to form numerous channels in bacterial membranes that interfere with membrane functions, resulting in cell death. © Oxford University Press Regular Papers nationallicence active transport nationallicence antimicrobial peptide nationallicence ATP synthesis nationallicence D-enantiomer nationallicence membrane potential nationallicence Alvarez-Bravo Juan Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 aut Kurata Shoichiro Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 aut Natori Shunji Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1312-1316 0021-924X 117:6<1312 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124860 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124860 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alvarez-Bravo Juan Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Kurata Shoichiro Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Natori Shunji Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1312-1316 0021-924X 117:6<1312 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford