caa a22 4500 397494491 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124841 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124841 Mitochondrial Alcohol Dehydrogenase from Ethanol-Grown Euglena gracilis [Elektronische Daten] [Kouki Ono, Yukari Kawanaka, Yuri Izumi, Hiroshi Inui, Kazutaka Miyatake, Shozaburo Kitaoka, Yoshihisa Nakano] The inducing effects of ethanol on alcohol dehydrogenase and the key enzymes of the glyoxylate cycle, isocitrate lyase and malate synthase, in Euglena cells were investigated. Ethanol as the sole carbon source resulted in increases in alcohol dehydrogenase and the two glyoxylate cycle enzymes. The experimental results indicated that ethanol is assimilated by alcohol dehydrogenase and the glyoxylate cycle in Euglena. Mitochondria from aerobically grown Euglena contain a unique type of alcohol dehydrogenase that accounts for their ability to respire with ethanol as a substrate. This alcohol dehydrogenase was purified to homogeneity from ethanol-grown Euglena gracilis. The mitochondrial alcohol dehydrogenase was NAD+-specific but not NADP+-specific. Ethanol was the most active substrate, but the enzyme was also active towards 1-butanol, 1-heptanol, cinnamyl alcohol, and myristyl alcohol. These results indicated that mitochondrial alcohol dehydrogenase participated in alcohol metabolism in Euglena gracilis. © Oxford University Press Regular Papers nationallicence alcohol dehydrogenase nationallicence ethanol metabolism nationallicence glyoxylate cycle nationallicence mitochondria nationallicence Protozoa nationallicence Ono Kouki Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut Kawanaka Yukari Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut Izumi Yuri Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut Inui Hiroshi Department of Agricultural Biochemistry and Biotechnology, Tottori University Tottori, Tottori 680 aut Miyatake Kazutaka Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut Kitaoka Shozaburo Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut Nakano Yoshihisa Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1178-1182 0021-924X 117:6<1178 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124841 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124841 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ono Kouki Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Kawanaka Yukari Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Izumi Yuri Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Inui Hiroshi Department of Agricultural Biochemistry and Biotechnology, Tottori University Tottori, Tottori 680 aut NATIONALLICENCE 700 1- Miyatake Kazutaka Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Kitaoka Shozaburo Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Nakano Yoshihisa Department of Applied Biological Chemistry, University of Osaka Prefecture Sakai, Osaka 593 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1178-1182 0021-924X 117:6<1178 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford