caa a22 4500 397494505 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199501 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124709 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124709 Involvement of Pertussis Toxin-Sensitive Mechanism in Retinoic Acid-Induced Differentiation of Human Leukemic HL-60 Cells [Elektronische Daten] [Yoshiharu Ohoka, Kenji Kontani, Taroh Iiri, Hiroshi Nishina, Toshiaki Katada] Retinoic acid-induced differentiation of human leukemic HL-60 cells is accompanied with the early induction of an ecto-enzyme of NAD+ glycohydrolase (NADase), which has recently been identified as human leukocyte cell surface antigen CD38 [Kontani, K. et al. (1993) J. Biol. Chem. 268, 16895-16898]. The terminal cell differentiation attendant upon the cell growth arrest was, but the early induction of CD38 NADase activity was not, inhibited by prior treatment of HL-60 cells with pertussis toxin, which catalyzed ADP-ribosylation of the membrane-bound αβγ-trimeric GTP-binding proteins. The prior treatment was, however, not essential for the toxin-induced inhibition of the cell differentiation; the inhibition by the addition of pertussis toxin was still observed even after retinoic acid-induced expression of CD38 antigen. This suggested that a pertussis toxin-sensitive mechanism was involved in a late process of cell differentiation. Indeed, HL-60 cells appeared to secrete a differentiation-supporting factor in response to retinoic acid, since the cell differentiation was accelerated and potentiated upon culture of the cells in a conditioned medium prepared from retinoic acid-treated cells. The action of the differentiation-supporting factor was destroyed by heating and markedly attenuated in pertussis toxin-pretreated HL-60 cells. Thus, the whole process of the retinoic acid-induced cell differentiation appeared to consist of two distinguishable periods in terms of sensitivity to pertussis toxin; the toxin-insensitive early period characterized by the induction of CD38 NADase activity and the toxin-sensitive late period in which the secretion of a differentiation-supporting factor might be involved. © 1995 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence CD38 antigen nationallicence cell differentiation nationallicence GTP-binding proteins nationallicence pertussis toxin nationallicence retinoic acid nationallicence Ohoka Yoshiharu Department of Life Science, Tokyo Institute of Technology Midori-ku, Yokohama 227 aut Kontani Kenji Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut Iiri Taroh Department of Life Science, Tokyo Institute of Technology, Midori-ku, Yokohama 227, Hongo, Bunkyo-ku, Tokyo 113 aut Nishina Hiroshi Department of Life Science, Tokyo Institute of Technology Midori-ku, Yokohama 227 aut Katada Toshiaki Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 117/1(1995-01), 190-196 0021-924X 117:1<190 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124709 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124709 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohoka Yoshiharu Department of Life Science, Tokyo Institute of Technology Midori-ku, Yokohama 227 aut NATIONALLICENCE 700 1- Kontani Kenji Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Iiri Taroh Department of Life Science, Tokyo Institute of Technology, Midori-ku, Yokohama 227, Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Nishina Hiroshi Department of Life Science, Tokyo Institute of Technology Midori-ku, Yokohama 227 aut NATIONALLICENCE 700 1- Katada Toshiaki Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/1(1995-01), 190-196 0021-924X 117:1<190 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford