caa a22 4500 397494513 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124837 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124837 Morphology of a Cytochrome c-Adsorbed Stearic Acid Monolayer on Brewster Angle Microscopy [Elektronische Daten] [Hideaki Tanaka, Ken-ichi Hayashi, Takahisa Akatsuka, Jiro Toyama, Kazuyuki Noda, Tatsunori Kida, Yoshiro Ogoma, Toshihiro Fujii, Yoshiyuki Kondo] The morphologies of stearic acid and cytochrome c (cyt.c)-adsorbed stearic acid monolayers were investigated by Brewster angle microscopy (BAM) with various molecular areas of stearic acid. With an area of more than 0.38 nm2/molecule, many blight island domains and some bright circles were observed in the BAM image of the stearic acid monolayer. The blight site part became to occupy all the surface with compression, and then became more closely packed with an area of 0.22 nm2/molecule. On the other hand, a different BAM image was obtained for the cyt.c-adsorbed stearic acid monolayer, as follows: (i) a striped pattern was only observed in the presence of cyt.c; (ii) the number of bright circles in the presence of cyt.c was less than that in its absence. Furthermore, when a uniform BAM image was observed for the stearic acid monolayer with cyt.c, the intensity of the absorbance at 409 nm of cyt.c was the highest. By calculating the amount of cyt.c adsorbed on a stearic acid monolayer from the absorbance value, it was shown that cyt.c was most closely packed when an uniform BAM image was observed. These results suggest that the use of BAM and visible absorption spectroscopy together is useful for studying the morphology of a monolayer. © Oxford University Press Regular Papers nationallicence Brewster angle microscopy nationallicence cytochrome c nationallicence monolayer nationallicence morphology nationallicence stearic acid nationallicence Tanaka Hideaki Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut Hayashi Ken-ichi Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut Akatsuka Takahisa Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut Toyama Jiro Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut Noda Kazuyuki Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut Kida Tatsunori Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut Ogoma Yoshiro Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut Fujii Toshihiro Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut Kondo Yoshiyuki Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1151-1155 0021-924X 117:6<1151 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124837 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124837 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka Hideaki Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut NATIONALLICENCE 700 1- Hayashi Ken-ichi Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut NATIONALLICENCE 700 1- Akatsuka Takahisa Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut NATIONALLICENCE 700 1- Toyama Jiro Nippon Mektron Ltd. Kukizaki, Ibaraki 300-12 aut NATIONALLICENCE 700 1- Noda Kazuyuki Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut NATIONALLICENCE 700 1- Kida Tatsunori Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut NATIONALLICENCE 700 1- Ogoma Yoshiro Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut NATIONALLICENCE 700 1- Fujii Toshihiro Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut NATIONALLICENCE 700 1- Kondo Yoshiyuki Department of Functional Polymer Science, Faculty of Textile Science and Technology, Shinshu University Ueda, Nagano 386 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/6(1995-06), 1151-1155 0021-924X 117:6<1151 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford