caa a22 4500 397494521 CHVBK 20180308164517.0 cr unu---uuuuu 161202e199503 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124746 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124746 Crystal Structure Determination of a Flavoprotein FP390 from a Luminescent Bacterium, Photobacterium phosphoreum [Elektronische Daten] [Akiko Kita, Sabu Kasai, Kunio Miki] The three-dimensional structure of a flavoprotein, FP390, purified from a luminescent bacterium, Photobacterium phosphoreum, has been determined at 3 Â resolution by X-ray crystallography. Crystallographic refinements of the structural model have led to an R-factor of 0.24 for the intensity data between 6 to 3 Â resolution collected with synchrotron radiation. It was found that a homodimer of the FP390 molecules related by a non-crystallo-graphic 2-fold axis is comprised in the asymmetric unit. Two homodimers are arranged around a crystallographic 2-fold axis to form a tetrameric assembly. The monomer molecule of FP390, to which two molecules of the flavin cofactor (Q-flavin) are bound, consists of a seven-stranded parallel β-sheet which forms a half of the β-barrel structure and seven α-helices which surround one side of the β-barrel. We suggest that the reason why the Q-flavin sample prepared from FP390 is always a mixture of two components is connected with the fact that the monomer molecules has two flavin binding sites, at the dimer interface and at the molecular surface. © 1995 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence crystal structure nationallicence FP390 nationallicence luxF nationallicence Q-flavin nationallicence Photobacterium phosphoreum nationallicence Kita Akiko Research Laboratory of Resources Utilization, Tokyo Institute of Technology Nagatsuta, Midori-ku, Yokohama 227 aut Kasai Sabu Department of Bioapplied Chemistry, Faculty of Engineering, Osaka City University Sugimoto, Sumiyoshi-ku, Osaka 558 aut Miki Kunio Research Laboratory of Resources Utilization, Tokyo Institute of Technology Nagatsuta, Midori-ku, Yokohama 227 aut The Journal of Biochemistry Oxford University Press 117/3(1995-03), 575-578 0021-924X 117:3<575 1995 117 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124746 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124746 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kita Akiko Research Laboratory of Resources Utilization, Tokyo Institute of Technology Nagatsuta, Midori-ku, Yokohama 227 aut NATIONALLICENCE 700 1- Kasai Sabu Department of Bioapplied Chemistry, Faculty of Engineering, Osaka City University Sugimoto, Sumiyoshi-ku, Osaka 558 aut NATIONALLICENCE 700 1- Miki Kunio Research Laboratory of Resources Utilization, Tokyo Institute of Technology Nagatsuta, Midori-ku, Yokohama 227 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 117/3(1995-03), 575-578 0021-924X 117:3<575 1995 117 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford