caa a22 4500 39749453X CHVBK 20180308164517.0 cr unu---uuuuu 161202e199507 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a124891 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a124891 Intracellular Sorting of Lysosomal β-Glucuronidase Is Altered Due to Administaration of Dibutyl Phosphate [Elektronische Daten] [Yukio Nishimura, Keitaro Kato, Kimimitsu Oda, Toyoko Ishikawa, Yukio Ikehara, Masaru Himeno] Organophosphate compounds are known to cause a selective increase of β-glucuronidase activity in rat serum. Previous data suggested that increase of serum β-glucuronidase activity was well correlated with decrease of that activity in rat liver microsomal fraction, thereby, suggesting a role of the microsomal enzyme in mediating the organophosphate effect. To investigate further the intracellular sorting pathway of β-glucuronidase in dibuty 1 phosphate-treated rats, liver subcellular fractions were prepared at 12 or 48 h after in vivo administration of [3H]leucine and it was established that microsomal β- glucuron-idase was the origin of the increased serum enzyme. To characterize the intracellular secretory pathway of β-glucuronidase in dibutyl phosphate-treated rats, Golgi subfrac-tions were isolated and a time course study was carried out. At 30 min after administration of dibutyl phosphate, specific activity of β-glucuronidase in GF-2 (Golgi intermediate fraction) and GF-3 (Golgi heavy fraction) was significantly increased to the maximum. Furthermore, colchicine pretreatment of rats caused a delay of the peak of specific activity for 30 min in GF-2 and GF-3, and accumulation of enzyme activity in Golgi subtractions was observed. Colchicine pretreatment also had an inhibitory effect on release of β-glu-curonidase into serum until 30 min after dibutyl phosphate injection. The electrophoretic pattern of microsomal β-glucuronidase on polyacrylamide gel was found to show two major bands of microsomal enzyme type and lysosomal enzyme type in dibutyl phosphate-treated rats. Taken together, these findings indicate that microsomal β-glucuronidase follows the intracellular secretory pathway and is secreted into serum via Golgi complex in response to dibutyl phosphate. © by the Journal of Biochemistry Regular Papers nationallicence dibutyl phosphate nationallicence βglucuronidase nationallicence Golgi complex nationallicence lysosomes nationallicence protein transport nationallicence Nishimura Yukio Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut Kato Keitaro Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut Oda Kimimitsu Niigata University School of Dentistry Niigata 951 aut Ishikawa Toyoko Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut Ikehara Yukio Department of Biochemistry, Fukuoka University School of Medicine Jonan-ku, Fukuoka 814-01 aut Himeno Masaru Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut The Journal of Biochemistry Oxford University Press 118/1(1995-07), 46-55 0021-924X 118:1<46 1995 118 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a124891 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a124891 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishimura Yukio Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut NATIONALLICENCE 700 1- Kato Keitaro Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut NATIONALLICENCE 700 1- Oda Kimimitsu Niigata University School of Dentistry Niigata 951 aut NATIONALLICENCE 700 1- Ishikawa Toyoko Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut NATIONALLICENCE 700 1- Ikehara Yukio Department of Biochemistry, Fukuoka University School of Medicine Jonan-ku, Fukuoka 814-01 aut NATIONALLICENCE 700 1- Himeno Masaru Division of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University Higashi-ku Fukuoka 812-82 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 118/1(1995-07), 46-55 0021-924X 118:1<46 1995 118 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford