caa a22 4500 397496362 CHVBK 20180308164522.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1016/S1010-7940(05)80129-0 doi (NATIONALLICENCE)oxford-10.1016/S1010-7940(05)80129-0 Myocardial protection with and without leukocyte depletion: a comparative study on the oxidative stress [Elektronische Daten] We tested the hypothesis that controlled reperfusion with leukocyte-depleted blood could improve myocardial protection by reducing theoxidative stress in patients undergoing myocardial revascularization.Thirty-four patients receiving antegrade/retrograde blood cardioplegia weredivided into: group A: 11 patients with ejection fractions (EF) less than35%, treated with leukocyte-depleted controlled blood reperfusion, group B:11 patients with EF less than 35% in whom no leukocyte depletion wasperformed, group C: 6 patients with EF more than 45% treated as group A andgroup D: 6 patients with EF more than 45% without leukocyte depletion. Toasses the oxidative stress, we evaluated total, total oxidized (GSSX), andreduced glutathione (GSH) in coronary sinus plasma, immediately beforecross-clamping the aorta (T0), and at 0 (T1), 15 (T2) and 30 (T3) min afterunclamping it. In groups A and B a significant shift towards oxidation ofredox status of glutathione (GSH/GSSX) at T1 vs T0 was observed.Glutathione redox ratio remained low in group B while in group A itreturned to the basal value at T2 with a significant difference from groupB at T2 and T3. No differences were observed between groups C and D. Inconclusion, our data show that leukocyte-depleted reperfusion can afford abetter myocardial protection in patients with left ventricular dysfunction,while it seems unnecessary in patients with normal EF. © Springer-Verlag European Journal of Cardio-Thoracic Surgery Elsevier Science B.V. 9/12(1995-12), 701-706 1010-7940 9:12<701 1995 9 ejcts https://doi.org/10.1016/S1010-7940(05)80129-0 text/html Onlinezugriff via DOI 1 abstract jats NATIONALLICENCE 856 40 https://doi.org/10.1016/S1010-7940(05)80129-0 text/html Onlinezugriff via DOI NATIONALLICENCE 773 0- European Journal of Cardio-Thoracic Surgery Elsevier Science B.V 9/12(1995-12), 701-706 1010-7940 9:12<701 1995 9 ejcts Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford