caa a22 4500 397520506 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199503 xx s 000 0 eng 10.1093/jxb/46.3.329 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.3.329 The influence of the embryonic axis and cytokinins on reserve mobilization in germinating lupin seeds [Elektronische Daten] [Shyamal K. Nandi, Lok Man S. Palni, Geert J.M. de Klerk] The influence of the embryonic axis and cytokinins (CKs) on reserve mobilization has been examined in yellow lupin (Lupinus luteus L. cv. JSG 6167) seed during germination and during early growth for up to 9 d in the dark. The study included determination of starch, soluble sugars, proteins, and amino acid content. Amylolytic and proteolytic enzyme activity was also measured in untreated cotyledons with intact embryo (attached) or detached cotyledons (embryo removed), and in detached cotyledons following treatment with CKs namely, dihydrozeatin, (diH)Z, and 6-benzylaminopurine, BAP. Generally, the detached cotyledons showed reduced mobilization and decreased enzymatic activity in comparison to attached cotyledons, indicating the importance of the embryonic axis in this process. However, a rise in protease activity and free amino acid content was detected in 9-d-old detached cotyledons suggesting that the end products do not necessarily inhibit enzyme activity. While (diH)Z was partially effective in inducing reserve mobilization and enzymatic activity in detached cotyledons, the effect of BAP was more pronounced and appeared to replace the embryonic axis. The embryonic axis of this species has recently been shown to synthesize CKs which are transported to the cotyledons, are highly stabe and induce cotyledon expansion and chlorophyll synthesis. The results of the present investigation and previous studies from this laboratory collectively indicate that the regulation of reserve mobilization in yellow lupin seeds appears to be mediated, at least in part, by a stimulus, probably a CK, emanating from the embryonic axis. © Oxford University Press Research Papers nationallicence Lupinus luteus nationallicence cytokinins nationallicence benzylaminopurine nationallicence dihydrozeatin nationallicence embryonic axis nationallicence lupin seeds nationallicence reserve mobilization nationallicence Nandi Shyamal K. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra City, ACT 2601, Australia aut Palni Lok Man S. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra City, ACT 2601, Australia aut de Klerk Geert J.M. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra City, ACT 2601, Australia aut Journal of Experimental Botany Oxford University Press 46/3(1995-03), 329-336 0022-0957 46:3<329 1995 46 exbotj https://doi.org/10.1093/jxb/46.3.329 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.3.329 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nandi Shyamal K. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra City, ACT 2601, Australia aut NATIONALLICENCE 700 1- Palni Lok Man S. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra City, ACT 2601, Australia aut NATIONALLICENCE 700 1- de Klerk Geert J.M. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, Canberra City, ACT 2601, Australia aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/3(1995-03), 329-336 0022-0957 46:3<329 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford