caa a22 4500 397520581 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/jxb/46.10.1579 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.10.1579 Polypeptides associated with the induction of direct somatic embryogenesis in Camellia japonica leaves I. Identification of embryo-specific polypeptides [Elektronische Daten] [M. Cristina Pedroso, Jean-Louis Hilbert, Jacques Vasseur, M. Salomé Pais] Embryogenic and non-embryogenic induced leaves of Camellia japonica, with the same shoot origin and submitted to the same culture conditions, were used to study protein changes during the induction of direct embryogenesis. The analysis of protein changes in these samples, based on two-dimensional polyacrylamide gel electrophoresis, revealed that 91% and 66.2% of the polypeptides detected, respectively, in embryogenic and non-embryogenic induced leaves, were already present in the non-induced control leaves. The results of the differential expression of eight selected polypeptides detected in induced and non-induced leaves are presented. A spotlist report was obtained for the 9% (43) embryogenesis-associated leaf polypeptides. From these polypeptides, two polypeptides were identified which seem to be specifically associated with somatic embryogenesis in C. japonica: E1 (pl 5.6; mol. wt. 43.5) and E2 (pl 6.0; mol. wt. 25.7). © Oxford University Press Research Papers nationallicence Camellia japonica nationallicence embryogenesis-associated polypeptides nationallicence somatic embryogenesis nationallicence two-dimensional polyacrylamide gel electrophoresis nationallicence Pedroso M. Cristina Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa Bloco C2, Piso 1, Campo Grande, P-1700 Lisboa, Portugal aut Hilbert Jean-Louis Laboratoire de Physiologie Cellulaire et de Morphogenèse Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq, France aut Vasseur Jacques Laboratoire de Physiologie Cellulaire et de Morphogenèse Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq, France aut Pais M. Salomé Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa Bloco C2, Piso 1, Campo Grande, P-1700 Lisboa, Portugal aut Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1579-1584 0022-0957 46:10<1579 1995 46 exbotj https://doi.org/10.1093/jxb/46.10.1579 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.10.1579 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pedroso M. Cristina Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa Bloco C2, Piso 1, Campo Grande, P-1700 Lisboa, Portugal aut NATIONALLICENCE 700 1- Hilbert Jean-Louis Laboratoire de Physiologie Cellulaire et de Morphogenèse Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq, France aut NATIONALLICENCE 700 1- Vasseur Jacques Laboratoire de Physiologie Cellulaire et de Morphogenèse Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq, France aut NATIONALLICENCE 700 1- Pais M. Salomé Departamento de Biologia Vegetal, Faculdade de Ciências, Universidade de Lisboa Bloco C2, Piso 1, Campo Grande, P-1700 Lisboa, Portugal aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1579-1584 0022-0957 46:10<1579 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford