caa a22 4500 397520603 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.special_issue.1325 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.special_issue.1325 Integration of cytosolic and plastidic carbon metabolism by fructose 2,6-bisphosphate [Elektronische Daten] [Nicholas J. Kruger, Peter Scott] The aim of this report is to assess the role of fructose 2,6-bisphosphate (Fru-2,6-P2) in integration of metabolism between the cytosol and chloroplast during photosynthesis. Transgenic plants expressing modified copies of the rat liver bifunctional 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were used to obtain lines differing specifically in their Fru-2,6-P2 concentrations. Such plants differed in the quantum requirement for photosynthesis, rate of oxygen evolution, and the extent of fluorescence quenching at the onset of illumination. Increasing Fru-2,6-P2 resulted in a progressive increase in the period and number of oscillations in oxygen evolution and fluorescence following dark-light transition in saturating carbon dioxide. At the beginning of the photoperiod, transgenic lines with increased amounts of Fru-2,6-P2 showed greater starch accumulation and reduced fluxes to soluble sugars, organic acids and amino acids. In contrast, transgenic lines with reduced Fru-2,6-P2 concentrations accumulated sucrose more rapidly than untransformed plants, while the rate of starch synthesis was lower. The flux response coefficients for Fru-2,6-P2 on sucrose and starch synthesis were — 0.43 and 0.33, respectively. These results are interpreted as evidence that Fru-2,6-P2 makes a significant contribution to the potential limitation of photosynthesis by sucrose production in the cytosol. © 1995 Oxford University Press Sucrose Synthesis nationallicence Nicotiana tabacum nationallicence fructose 2,6-bisphosphate nationallicence photosynthesis nationallicence sucrose synthesis nationallicence carbon partitioning nationallicence Kruger Nicholas J. Department of Plant Sciences, University of Oxford, Oxford 0X1 3RB, UK aut Scott Peter Department of Plant Sciences, University of Oxford, Oxford 0X1 3RB, UK aut Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1325-1333 0022-0957 46:special_issue<1325 1995 46 exbotj https://doi.org/10.1093/jxb/46.special_issue.1325 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.special_issue.1325 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kruger Nicholas J. Department of Plant Sciences, University of Oxford, Oxford 0X1 3RB, UK aut NATIONALLICENCE 700 1- Scott Peter Department of Plant Sciences, University of Oxford, Oxford 0X1 3RB, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1325-1333 0022-0957 46:special_issue<1325 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford