caa a22 4500 397520611 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.9.1093 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.9.1093 Expansion of pea leaves subjected to short water deficit: cell number and cell size are sensitive to stress at different periods of leaf development [Elektronische Daten] [Jérémie Lecoeur, Jacques Wery, Olivier Turc, François Tardieu] We have followed the expansion of individual pea leaves from initiation to maximum area, over two markedly different periods. During the first one (2/3 of total leaf development time), cell production occurred while cell and leaf expansions were slow. Rapid expansion (95% of total) occurred for a second period lasting 1/3 of total development time, whereas cell division was virtually completed. Water deficits of 15 d were applied during either slow or rapid expansion, and characterized by measurements of soil water potential, stomatal conductance, leaf water potential and xylem [ABA]. Plants which experienced water deficit during the slow expansion period had markedly reduced expansion during the second period (i.e. 1 or 2 weeks after cessation of deficit), while all variables characterizing water status were returned to the level of the control. This ‘after effect' was accounted for by a reduced cell number per leaf, while individual cell area was not affected. In contrast, water deficit occurring during rapid leaf expansion immediately reduced leaf expansion via cell area, without affecting cell number per leaf. These experiments indicate a role, in the response to water deficits, for events occurring very early in the development of pea leaves, while leaf expansion is too slow to be measured with macroscopic methods. This role would be accounted for by cell production during the first 2/3 of leaf development while cell expansion would account for changes in the area of leaves experiencing a later stress. These results suggest that long-term temporal analysis may be essential in the study of dicot leaf expansion compared to monocot leaves where temporal analysis can be inferred from spatial analysis. © Oxford University Press RESEARCH PAPERS nationallicence Leaf growth nationallicence dicot leaves nationallicence water stress nationallicence ell division nationallicence cell expansion nationallicence Pisum sativum L nationallicence Lecoeur Jérémie INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut Wery Jacques INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut Turc Olivier INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut Tardieu François INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1093-1101 0022-0957 46:9<1093 1995 46 exbotj https://doi.org/10.1093/jxb/46.9.1093 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.9.1093 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lecoeur Jérémie INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut NATIONALLICENCE 700 1- Wery Jacques INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut NATIONALLICENCE 700 1- Turc Olivier INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut NATIONALLICENCE 700 1- Tardieu François INRA-ENSA.M, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux (LEPSE), 2, Place Viala, F-34060 Montpellier cedex 01, France aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1093-1101 0022-0957 46:9<1093 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford