caa a22 4500 397520646 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jxb/46.2.255 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.2.255 Effect of calcium on callus friability and somatic embryogenesis in Hevea brasiliensis Müll. Arg.: relations with callus mineral nutrition, nitrogen metabolism and water parameters [Elektronische Daten] [P. Montoro, H. Etienne, M.P. Carron] Changes in culture medium calcium content modified the texture, embryo development, and water and mineral status of Hevea brasiliensis (Müll. Arg.) calli. With concentrations of 0 to 9 mM CaCl2 in the callogenesis medium, high percentages of embryogenic calli were obtained with only a slight change in their compactness. Higher concentrations led to friable calli whose ability to produce embryos then depended on low calcium concentrations in the embryogenesis medium; in effect, only low calcium contents allowed embryo development. Given that 12 mM CaCl2 in the first culture medium resulted in friable calli, it was assumed that there was an interaction between the calcium and growth regulators in the medium. The change in texture and morphogenetic ability was combined with a drop in turgidity potential and water availability, despite an increase in water content. This change was also associated with a change in mineral nutrition, notably a drop in nitrate uptake, combined with an increase in osmotic potential and a drop in protein synthesis. © Oxford University Press Research Papers nationallicence Calcium nationallicence somatic embryogenesis nationallicence friability nationallicence nitrogen metabolism nationallicence water status nationallicence Montoro P. CIRAD-CP, Programme Hévéa, Unité BIOTROP, BP 5035, F-34032 Montpellier cedex, France aut Etienne H. CIRAD-CP, Programme Hévéa, Unité BIOTROP, BP 5035, F-34032 Montpellier cedex, France aut Carron M.P. CIRAD-CP, Programme Hévéa, Unité BIOTROP, BP 5035, F-34032 Montpellier cedex, France aut Journal of Experimental Botany Oxford University Press 46/2(1995-02), 255-261 0022-0957 46:2<255 1995 46 exbotj https://doi.org/10.1093/jxb/46.2.255 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.2.255 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montoro P. CIRAD-CP, Programme Hévéa, Unité BIOTROP, BP 5035, F-34032 Montpellier cedex, France aut NATIONALLICENCE 700 1- Etienne H. CIRAD-CP, Programme Hévéa, Unité BIOTROP, BP 5035, F-34032 Montpellier cedex, France aut NATIONALLICENCE 700 1- Carron M.P. CIRAD-CP, Programme Hévéa, Unité BIOTROP, BP 5035, F-34032 Montpellier cedex, France aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/2(1995-02), 255-261 0022-0957 46:2<255 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford