caa a22 4500 397520670 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/jxb/46.10.1525 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.10.1525 Influence of abscisic acid on nitrogen partitioning, sucrose metabolism and nitrate reductase activity of chicory suspension cells [Elektronische Daten] [Abdelali Chraibi, Benoît Paîms, Nathalie Druart, Pascale Goupil, Alain Gojon, Serge Rambour] Batch suspension cultures of chicory cells (Cichorium intybus L. var. Witloof) possess a NADH-specific nitrate reductase activity that peaks on day 3 of a 10 d growth cycle. When both nitrate and ammonium are used as nitrogen sources, chicory cells absorb nitrate irst. Ammonium uptake becomes predominant at day 3, even though NO3− was still present in the medium. Although abscisic acid impairs growth as well as 15NO3− uptake and reduction, it promotes nitrate reductase activity as measured both in vivo and in vitro. Specific activity is 50% higher in ABA-treated cells than in controls. These conflicting data may be explained either in erms of nitrate reductase levels or by the availability of reducing power and energy. Since NRA is generally controlled by the availability of the reducing power, the energy status of the cell, the adenylate nucleotide pools, were measured simultaneously with the carbohydrate levels within the cell and the growth medium. The energy charge was not modified during the growth cycle, regardless of the rowth conditions. Yet ABA modified the intracellular carbohydrate metabolism and inhibited the acidic invertase, the sucrose synthase and the sucrose phosphate synthase activities. Modified assimilation rates of nitrate in chicory cells grown in the presence of ABA, were probably correlated to modified carbohydrate metabolism pathways leading to increased availability of reducing power, energy and C-skeletons. © Oxford University Press Research Papers nationallicence Abscisic acid nationallicence Cichorium intybus L nationallicence nitrate reductase nationallicence reductase nationallicence invertase nationallicence sucrose synthase nationallicence sucrose phosphate synthase nationallicence Chraibi Abdelali Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut Paîms Benoît Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut Druart Nathalie Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut Goupil Pascale Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut Gojon Alain Laboratoire de Biochimie et Physiologie Végétales, INRA/ENSAM, CNRS URA 573 F-34060 Montpellier Cedex, France aut Rambour Serge Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1525-1533 0022-0957 46:10<1525 1995 46 exbotj https://doi.org/10.1093/jxb/46.10.1525 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.10.1525 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chraibi Abdelali Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Paîms Benoît Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Druart Nathalie Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Goupil Pascale Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 700 1- Gojon Alain Laboratoire de Biochimie et Physiologie Végétales, INRA/ENSAM, CNRS URA 573 F-34060 Montpellier Cedex, France aut NATIONALLICENCE 700 1- Rambour Serge Physiologie et Génétique Moléculaire Végétates, Bat. SN 2, USTL F-59655 Villeneuve d'Ascq Cedex, France aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1525-1533 0022-0957 46:10<1525 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford