caa a22 4500 397520719 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/jxb/46.6.701 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.6.701 Physiological basis of pre-harvest sprouting resistance in Sorghum bicolor (L.) Moench. ABA levels and sensitivity in developing embryos of sprouting-resistant and -susceptible varieties [Elektronische Daten] [Haydée S. Steinbach, R.L. Benech-Arnold, Graciela Kristof, R.A. Sanchez, Susana Marcucci-Poltri] Pre-harvest sprouting is a major problem in Sorghum crops which leads to losses in seed viability and produces important decreases in grain weight. In this paper we aimed to have an insight into the physiological basis of pre-harvest sprouting resistance in this crop by assessing germinability, ABA embryonic content and embryonic sensitivity to ABA during seed development in three varieties presenting contrasting sprouting behaviour: Redland B2 (very susceptible), SC 650 (moderately resistant) and IS 9530 (very resistant). Redland B2 caryopses were able to germinate with high germination indices from early stages of development, while caryopses from IS 9530 did not present germination indices different from 0 until near physiological maturity. SC 650 (moderately resistant) grains presented an intermediate pattern of behaviour. In all three varieties isolated embryos were able to germinate with maximum germination indices from as early as 15 d after pollination (DAP). Differences in grain dormancy level were not paralleled by a consistently different endogenous ABA content throughout maturation. However, when ABA embryonic content was measured in incubated 35 DAP caryopses, ABA level in B2 embryos after 24 h of incubation was found to be less than half that observed in IS 9530 embryo after the same period of incubation. In addition, B2 embryos were found to be 10-fold less sensitive to the inhibitory effect of ABA than embryos from the other two varieties. These results explain to a considerable extent differences in germinability between sproutingresistant and -susceptible varieties and are consistent with differences in sprouting behaviour. © Oxford University Press Research Papers nationallicence Sorghum bicolor nationallicence abscisic acid nationallicence pre-harvest sprouting nationallicence seed development nationallicence germination nationallicence Steinbach Haydée S. cátedra de Cerealicultura, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut Benech-Arnold R.L. cátedra de Cerealicultura, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut Kristof Graciela cátedra de Cerealicultura, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut Sanchez R.A. Cátedra de Fisiologfa Vegetal, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut Marcucci-Poltri Susana INTA, Castelar, Argentina aut Journal of Experimental Botany Oxford University Press 46/6(1995-06), 701-709 0022-0957 46:6<701 1995 46 exbotj https://doi.org/10.1093/jxb/46.6.701 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.6.701 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Steinbach Haydée S. cátedra de Cerealicultura, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Benech-Arnold R.L. cátedra de Cerealicultura, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Kristof Graciela cátedra de Cerealicultura, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Sanchez R.A. Cátedra de Fisiologfa Vegetal, Facultad de Agronomia, Universidad de Buenos Aires Avenida San Martin 4453, 1417 Buenos Aires, Argentina aut NATIONALLICENCE 700 1- Marcucci-Poltri Susana INTA, Castelar, Argentina aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/6(1995-06), 701-709 0022-0957 46:6<701 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford