caa a22 4500 397520727 CHVBK 20180308164631.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.special_issue.1269 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.special_issue.1269 Engineering Rubisco to change its catalytic properties [Elektronische Daten] [Graeme Bainbridge, Pippa Madgwick, Saroj Parmar, Rowan Mitchell, Matthew Paul, Jim Pitts, Alfred J. Keys, Martin A.J. Parry] The initial steps of carbon assimilation and photorespiration are catalysed by ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39). Natural variation in the kinetic properties of the enzyme suggest that it is possible to alter the enzyme to favour the carboxylation activity relative to oxygenation. Mutagenesis in vitro of the gene encoding the large subunit of the enzyme from Anacystis nidulans has been used to modify catalytic properties. Residues at the C-terminal end of loop 6 of the β/α barrel structure of the large subunit influence specificity towards the gaseous substrates, CO2 and O2. None of the residues altered by mutagenesis appear to interact directly with the transition state analogue and their effect on the reaction of the enediolate intermediate with the gaseous substrates and stabilization of the resulting transition state intermediates by lysine 334 must be indirect. Interactions with other parts of the enzyme must also be important in determining substrate specificity. Backbone carbonyl groups close to lysine 334 interact with lysine 128; mutation of lysine 128 to residues of less positive polarity reduces enzyme activity and favours oxygenation relative to carboxylation. The likely effects on assimilation rates of altering the kinetic properties of Rubisco have been modelled. A leaf with cyanobacterial Rubisco may out-perform a higher plant Rubisco at elevated CO2 and cool temperatures. © 1995 Oxford University Press Photosynthetic Carbon Reduction nationallicence Anacystis nidulans nationallicence Rubisco nationallicence specificity factor nationallicence Bainbridge Graeme Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Madgwick Pippa Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Parmar Saroj Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Mitchell Rowan Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Paul Matthew Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Pitts Jim Crystallography Department, Birkbeck College, University of London Malet Street, London WC1E 7HX, UK aut Keys Alfred J. Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Parry Martin A.J. Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1269-1276 0022-0957 46:special_issue<1269 1995 46 exbotj https://doi.org/10.1093/jxb/46.special_issue.1269 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.special_issue.1269 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bainbridge Graeme Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 700 1- Madgwick Pippa Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 700 1- Parmar Saroj Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 700 1- Mitchell Rowan Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 700 1- Paul Matthew Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 700 1- Pitts Jim Crystallography Department, Birkbeck College, University of London Malet Street, London WC1E 7HX, UK aut NATIONALLICENCE 700 1- Keys Alfred J. Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 700 1- Parry Martin A.J. Biochemistry and Physiology Department, IACR-Rothamsted Harpenden, Herts ALS 2JQ, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1269-1276 0022-0957 46:special_issue<1269 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford