caa a22 4500 397520786 CHVBK 20180308164632.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/jxb/46.6.647 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.6.647 The free space of sugars in plant tissues6 [Elektronische Daten] [Axel Fleischer, Rudolf Ehwald] The total volume of the free space (FS) and the external film (EF) outside the cell wall of living plant materials was determined as partition volumes of sugars and dextran, respectively. Sugar uptake and kinetics of FS and EF saturation were recorded polarimetrically. The FS equilibrates within 20 s in suspension culture of Chenopodium album L. Here, the free apoplastic volume (FAV), i.e. the difference between sugar and dextran partition volumes, was determined by shortterm partitioning of fructose and dextran in samples containing the plant material and an optically compensated (non-rotating) solution of both components. The selective partitioning of the sugar with the FAV causes an increase in optical activity, which is dependent on the FAV. The FAV of a suspension cultured biomass was found to be correlated with the percentage volume of dead cells. The volume originating by plasmolysis between cell wall and plasma membrane was measured as an increase of the FAV. In seedling root segments of Zea mays L., the component of FS equilibrating within 1 min consisted of the EF and a portion of the FAV (probably dead cells at the surface). Saturation of the apoplast of the infiltrated root cortex with α-methylglucoside, a sugar not taken up by the cells, showed slow equilibration kinetics and was not complete within 30 min. © Oxford University Press Research Papers nationallicence Suspension culture nationallicence roots nationallicence apoplastic volume nationallicence viability nationallicence plasmolysis nationallicence Fleischer Axel Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, Institut für Biologie, Invalidenstrasse 42, D-10115 Berlin, Germany aut Ehwald Rudolf Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, Institut für Biologie, Invalidenstrasse 42, D-10115 Berlin, Germany aut Journal of Experimental Botany Oxford University Press 46/6(1995-06), 647-654 0022-0957 46:6<647 1995 46 exbotj https://doi.org/10.1093/jxb/46.6.647 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.6.647 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fleischer Axel Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, Institut für Biologie, Invalidenstrasse 42, D-10115 Berlin, Germany aut NATIONALLICENCE 700 1- Ehwald Rudolf Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät I, Institut für Biologie, Invalidenstrasse 42, D-10115 Berlin, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/6(1995-06), 647-654 0022-0957 46:6<647 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford