caa a22 4500 397520891 CHVBK 20180308164632.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.9.1123 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.9.1123 Activated oxygen production and detoxification in wheat plants subjected to a water deficit programme [Elektronische Daten] [M. Menconi, C.L.M. Sgherri, C. Pinzino, F. Navari-lzzo] Wheat plants (Triticum durum L. cv. Ofanto) were grown in a controlled environment. In one set, control plants were regularly watered; the other set of plants was subjected to two water deficit periods obtained by withholding water and rewatering to field capacity at the end of the first period. After both periods of stress, water potential (Ψw), pressure potential (Ψp) and relative water content (RWC) decreased; osmotic potential (Ψπ decreased by 0.3 MPa only after the second period of stress. In both treatments no osmoregulation mechanism occurred, however, an elastic adjustment took place and turgor was maintained at positive levels. Following the first treatment the good functionality of the ascorbate/glutathione cycle allowed the plants to maintain hydrogen peroxide to the control level despite a greater capacity of the thylakoid membranes to leak electrons towards oxygen; moreover, the ascorbate (AsA)/dehydroascorbate (DHA) ratio was unchanged while reduced glutathione (GSH)/oxidized glutathione (GSSG) increased in comparison with the control. Following the second period of stress, the decreased enzyme defence activities of the glutathione reductase (GR), dehydroascorbate reductase (DHAR) and ascorbate peroxidase (AsAP) together with a minor glutathione content might be a consequence of a reduced rate of activated oxygen production. © Oxford University Press RESEARCH PAPERS nationallicence Ascorbate/glutathione cycle nationallicence hydrogen peroxide nationallicence superoxide radical nationallicence Triticum durum L nationallicence water status nationallicence Menconi M. Istituto di Chimica Agraria, Università degli Studi di Pisa Via S. Michele degli Scalzi, 2,1-56124 Pisa, Italy aut Sgherri C.L.M. Istituto di Chimica Agraria, Università degli Studi di Pisa Via S. Michele degli Scalzi, 2,1-56124 Pisa, Italy aut Pinzino C. Istituto di Chimica Quantistica ed Energetica Molecolare C.N.R., Via Risorgimento, 35,1-56126 Pisa, Italy aut Navari-lzzo F. Istituto di Chimica Agraria, Università degli Studi di Pisa Via S. Michele degli Scalzi, 2,1-56124 Pisa, Italy aut Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1123-1130 0022-0957 46:9<1123 1995 46 exbotj https://doi.org/10.1093/jxb/46.9.1123 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.9.1123 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menconi M. Istituto di Chimica Agraria, Università degli Studi di Pisa Via S. Michele degli Scalzi, 2,1-56124 Pisa, Italy aut NATIONALLICENCE 700 1- Sgherri C.L.M. Istituto di Chimica Agraria, Università degli Studi di Pisa Via S. Michele degli Scalzi, 2,1-56124 Pisa, Italy aut NATIONALLICENCE 700 1- Pinzino C. Istituto di Chimica Quantistica ed Energetica Molecolare C.N.R., Via Risorgimento, 35,1-56126 Pisa, Italy aut NATIONALLICENCE 700 1- Navari-lzzo F. Istituto di Chimica Agraria, Università degli Studi di Pisa Via S. Michele degli Scalzi, 2,1-56124 Pisa, Italy aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1123-1130 0022-0957 46:9<1123 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford