caa a22 4500 397520905 CHVBK 20180308164632.0 cr unu---uuuuu 161202e199505 xx s 000 0 eng 10.1093/jxb/46.5.513 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.5.513 Phenylpropanoid defence responses in transgenic Lotus corniculatus [Elektronische Daten] II. Modelling plant defence responses in transgenic root cultures using thiol and carbohydrate elicitors [M.P. Robbins, B. Thomas, P. Morris] Transformed root cultures of Lotus corniculatus L. cv. Leo were treated with a range of thiol and carbohydrate elicitors. Both thiol reagents and fungal carbohydrate preparations resulted in an increase in the activity of phenylalanine ammonia lyase (PAL) in a concentration-dependent manner. One representative thiol elicitor, glutathione (GSH), and one fungal elicitor, derived from Rhynchosporium orthosporum autoclaved cell walls (Ro), were analysed in more detail. Both elicitors induced the transient accumulation of vestitol, an isoflavan phytoalexin, in tissue and in culture medium. Treatment of Lotus root cultures with the Ro elicitor resulted in a more rapid initial accumulation of this end product when compared with GSH, however, sativan (the 2-methoxy ester of vestitol) previously reported to co-accumulate in Lotus leaves was only detected following elicitation with high concentrations of GSH. Ro and GSH elicitors also induced the accumulation of a number of other phenylpropanoid compounds putatively identified as chalcones. The addition of thiol and carbohydrate elicitors to Lotus root cultures also resulted in characteristic changes in root morphology. Glutathione, in particular, resulted in the inhibition of root growth due to differential damage of meristem cells. © Oxford University Press Research Papers nationallicence Lotus corniculatus nationallicence hairy roots nationallicence elicitors nationallicence phytoalexins nationallicence Robbins M.P. Cell Manipulation Group, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK aut Thomas B. Cell Manipulation Group, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK aut Morris P. Cell Manipulation Group, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK aut Journal of Experimental Botany Oxford University Press 46/5(1995-05), 513-524 0022-0957 46:5<513 1995 46 exbotj https://doi.org/10.1093/jxb/46.5.513 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.5.513 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Robbins M.P. Cell Manipulation Group, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK aut NATIONALLICENCE 700 1- Thomas B. Cell Manipulation Group, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK aut NATIONALLICENCE 700 1- Morris P. Cell Manipulation Group, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/5(1995-05), 513-524 0022-0957 46:5<513 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford