caa a22 4500 39752093X CHVBK 20180308164632.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/jxb/46.10.1633 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.10.1633 The head structure of a higher plant V-type H+−ATPase is not always a hexamer but also a pentamer [Elektronische Daten] [Detlef Kramer, Bärbel Mangold, Angela Hille, llka Emig, Andreas Heβ, Rafael Ratajczak, Ulrich Lüttge] Pentameric head structures of the V-type H+−ATPase of Mesembryanthemum crystallinum L. were demonstrated in addition to hexameric head structures by rotational image analysis and molecular projections of negatively stained H+−ATPase heads. This observation, at least partially, is in contrast to the standard model of the V-type H+−ATPase predicting solely a hexameric head structure with three A and three B subunits in analogy to the F-type ATPases. With one A or B subunit missing two A or two B subunits would be adjacent to each other in the pentameric ATPase head. By chemical cross-linking of H +−ATPase subunits a crosslinking product exclusively consisting of B subunits, in addition to a cross-linking product consisting of subunits A and B was detected. Thus, the pentameric heads might lack one A subunit, although the lack of one B subunit can not be totally ruled out. We assume that the hexameric head structure is the catalytically active configuration while the pentameric head structure may be a relatively stable intermediate of turnover. © Oxford University Press Short Communications nationallicence V-type H+−ATPase nationallicence protein structure nationallicence electron microscopy nationallicence tonoplast nationallicence Mesembryanthemum crystallinum L nationallicence Kramer Detlef Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut Mangold Bärbel Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut Hille Angela Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut Emig llka Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut Heβ Andreas Abteilung für Akustik, Lernen und Sprache, Institut für Neurobiologie Brenneckestraβ 6, D-39118 Magdeburg, Germany aut Ratajczak Rafael Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut Lüttge Ulrich Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1633-1636 0022-0957 46:10<1633 1995 46 exbotj https://doi.org/10.1093/jxb/46.10.1633 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.10.1633 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kramer Detlef Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Mangold Bärbel Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Hille Angela Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Emig llka Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Heβ Andreas Abteilung für Akustik, Lernen und Sprache, Institut für Neurobiologie Brenneckestraβ 6, D-39118 Magdeburg, Germany aut NATIONALLICENCE 700 1- Ratajczak Rafael Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Lüttge Ulrich Institut für Botanik (FB 10), Technische Hochschule Darmstadt Schnittspahnstraβe 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1633-1636 0022-0957 46:10<1633 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford