caa a22 4500 397521006 CHVBK 20180308164632.0 cr unu---uuuuu 161202e199507 xx s 000 0 eng 10.1093/jxb/46.7.787 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.7.787 Glucosinolate biosynthesis in oilseed rape (Brassicanapus L.): studies with 35SO2−4 and glucosinolate precursors using oilseed rape pods and seeds [Elektronische Daten] [D. Toroser, C. Wood, H. Griffiths, D.R. Thomas] 35SO2−4 and glucosinolate precursors were administered to investigate the capacity of oilseed rape intact whole pods, isolated pod walls and seeds to carry out reactions of the glucosinolate biosynthetic pathway. Pod walls incorporated 35S-label from 35SO2−4 into both the thio-glucose and sulphonate-sulphur of glucosinolates. Pulse-labelling experiments showed that pod walls were the predominant source of the glucosinolates accumulated by the seeds. Isolated immatureseeds were capable of reductive assimilation of 35SO2−4 and incorporated 35S into sulphur-containing amino acids and the sulphonate moiety of glucosinolates, but were not able to incorporate 35S into the thio-glucose of glucosinolates. © Oxford University Press Research Papers nationallicence Biosynthesis nationallicence glucosinolates nationallicence oilseed rape nationallicence pod walls nationallicence seeds nationallicence Toroser D. The University of Newcastle upon Tyne, Department of Biological and Nutritional Sciences Newcastle upon Tyne NE1 7RU, UK aut Wood C. The University of Newcastle upon Tyne, Department of Biological and Nutritional Sciences Newcastle upon Tyne NE1 7RU, UK aut Griffiths H. The University of Newcastle upon Tyne, Department of Agricultural and Environmental Sciences Newcastleupon Tyne NE1 7RU, UK aut Thomas D.R. The University of Newcastle upon Tyne, Department of Biological and Nutritional Sciences Newcastle upon Tyne NE1 7RU, UK aut Journal of Experimental Botany Oxford University Press 46/7(1995-07), 787-794 0022-0957 46:7<787 1995 46 exbotj https://doi.org/10.1093/jxb/46.7.787 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.7.787 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toroser D. The University of Newcastle upon Tyne, Department of Biological and Nutritional Sciences Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 700 1- Wood C. The University of Newcastle upon Tyne, Department of Biological and Nutritional Sciences Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 700 1- Griffiths H. The University of Newcastle upon Tyne, Department of Agricultural and Environmental Sciences Newcastleupon Tyne NE1 7RU, UK aut NATIONALLICENCE 700 1- Thomas D.R. The University of Newcastle upon Tyne, Department of Biological and Nutritional Sciences Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/7(1995-07), 787-794 0022-0957 46:7<787 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford