caa a22 4500 397521014 CHVBK 20180308164632.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jxb/46.2.187 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.2.187 Symplastic communication between primary and developing lateral roots of Arabidopsis thaliana [Elektronische Daten] [Karl J. Oparka, Denton A.M. Prior, Kathryn M. Wright] The functional symplastic connections between primary and developing lateral roots of Arabidopsis were studied non-invasively using confocal laser scanning microscopy (CLSM), following ester-loading of the phloem with carboxyfluorescein (CF). Prior to the formation of lateral primordia in the pericycle, the phloem of the primary root behaved as an isolated conducting domain. However, the differentiation of phloem connector elements within the dividing pericycle allowed the rapid establishment of intercellular communication between the phloem and the cells of the lateral primordium. This communication was often established prior to the complete emergence of the lateral root from the parent root. Shortly after its emergence, functional conducting phloem became differentiated within the developing lateral root. A progressive isolation between the phloem and surrounding cells at the base of the lateral root was observed as the lateral continued to grow; the new phloem conducting CF to the elongation zone where it was unloaded symplastically from the protophloem into surrounding cells of the cortex and stele, a feature mirroring the pattern found near the apex of growing primary roots. Anomalous patterns of intercellular communication were found which indicated that previously functional symplastic pathways may have become sealed off following the emergence of some of the lateral roots. © Oxford University Press Research Papers nationallicence Arabidopsis nationallicence carboxyfluorescein nationallicence confocal laser scanning microscopy (CLSM) nationallicence intercellular transport nationallicence lateral roots nationallicence phloem (unloading) nationallicence symplast nationallicence Oparka Karl J. Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK aut Prior Denton A.M. Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK aut Wright Kathryn M. Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK aut Journal of Experimental Botany Oxford University Press 46/2(1995-02), 187-197 0022-0957 46:2<187 1995 46 exbotj https://doi.org/10.1093/jxb/46.2.187 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.2.187 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oparka Karl J. Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK aut NATIONALLICENCE 700 1- Prior Denton A.M. Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK aut NATIONALLICENCE 700 1- Wright Kathryn M. Department of Cellular and Environmental Physiology, Scottish Crop Research Institute, Invergowrie, Dundee DD25DA, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/2(1995-02), 187-197 0022-0957 46:2<187 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford