caa a22 4500 397521111 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199511 xx s 000 0 eng 10.1093/jxb/46.11.1745 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.11.1745 Effect of inhibition of protein glycosylation on auxin-induced growth and the occurrence of osmiophilic particles in maize (Zea mays L.) coleoptiles [Elektronische Daten] [Hans G. Edelmann, Rainer Bergfeld, Peter Schopfer] The dependence of auxin (IAA)-induced elongation growth on protein glycosylation was investigated in abraded maize (Zea mays L.) coleoptile segments, employing 2-deoxy-D-glucose (DOG) and tunicamycin (TUM) as inhibitors of protein glycosylation. TUM had no detectable effect on growth at 100μg ml−1. DOG impaired growth at concentrations larger than 1 mM. Total inhibition of growth occurred at a concentration of 20 mM. Similar effects were observed with mannose and glucosamine. The effect on wall-synthetic processes in the growth-limiting epidermis was analysed by tracer incorporation studies. Within 30 min hemicellulose and cellulose synthesis, measured as 3H-glucose incorporation, was not affected by DOG, indicating that inhibition of growth is not causally related to synthesis of both wall components. In contrast, protein synthesis and secretion into the walls, measured as incorporation of 3H-leucine into the TCA-precipitable protoplasmic and wall-bound protein, was rapidly inhibited by DOG. Concomitant with the effect on growth, DOG as well as mannose inhibited the occurrence of osmiophilic particles (OPs) which specifically occur at the growth-limiting epidermis during IAA-induced growth. The results provide evidence that IAA-induced wall loosening underlying elongation growth is dependent on O-glycosylation of proteins and their subsequent secretion into the epidermal walls. It appears that interference with these processes is responsible for inhibition of IAA induced growth by hexoses acting as anti-glucose antimetabolites. © Oxford University Press RESEARCH PAPERS nationallicence Auxin-induced growth nationallicence cell-wall synthesis nationallicence 2-deoxy-D-glucose nationallicence mannose nationallicence osmiophilic particles nationallicence tunicamycin nationallicence Edelmann Hans G. Botanisches Institut der Universität Bonn Venusbergweg 22, D-53115 Bonn, Germany aut Bergfeld Rainer Biologisches Institut II der Universität Freiburg Schänzlestr. 1, D-79104 Freiburg, Germany aut Schopfer Peter Biologisches Institut II der Universität Freiburg Schänzlestr. 1, D-79104 Freiburg, Germany aut Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1745-1752 0022-0957 46:11<1745 1995 46 exbotj https://doi.org/10.1093/jxb/46.11.1745 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.11.1745 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Edelmann Hans G. Botanisches Institut der Universität Bonn Venusbergweg 22, D-53115 Bonn, Germany aut NATIONALLICENCE 700 1- Bergfeld Rainer Biologisches Institut II der Universität Freiburg Schänzlestr. 1, D-79104 Freiburg, Germany aut NATIONALLICENCE 700 1- Schopfer Peter Biologisches Institut II der Universität Freiburg Schänzlestr. 1, D-79104 Freiburg, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1745-1752 0022-0957 46:11<1745 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford