caa a22 4500 397521189 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199511 xx s 000 0 eng 10.1093/jxb/46.11.1711 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.11.1711 Immunolabelling of cell surfaces of Arabidopsis thaliana roots following infection by Meloidogyne incognita (Nematoda) [Elektronische Daten] [M.J. Gravato Nobre, N. von Mende, L. Dolan, K.P. Schmidt, K. Evans, B. Mulligan] Studies of the migration of second stage juveniles (JJ2) of the root-knot nematode Meloidogyne incognita in Arabidopsis roots were made at the cellular level using immunolabelling techniques. A panel of antibodies that recognize epitopes present in the plant extracellular matrix (JIMs) and the nematode cuticle (PC245) were used. The normal route for the juvenile (J2) has been reconfirmed for both in vitro and in vivo conditions. Histological studies show that, during migration towards the root meristem, juveniles (JJ2) sometimes break the physico-chemical barrier of the endodermis and establish close contact with the central cylinder. Despite this, the juveniles continue their intercortical migration towards the root meristem. When the endodermis is breached, hyperplasia and hypertrophy occur and a premature gall is formed. Ultrastructural observations confirmed that loosening of the middle lamella occurs during progress through the cortex. Differences in the patterns of labelling of healthy and infected roots were revealed when the antipolygalacturonic acid antibody, JIM5, was applied; epitopes recognized by this antibody are mainly located on the triple junctions between cells. Some of the antibodies used proved very useful in illustrating the intercellular migration of JJ2 in the vascular cylinder, where they move in the vicinity of the protoxylem and future metaxylem cells. An envelope surrounding the nematodes, but located specifically on plant cell walls, was observed when infected rootsections were probed with PC245. This material at this interface appears to be of nematode origin. Characterization of the molecules involved is currently under investigation. © Oxford University Press RESEARCH PAPERS nationallicence Meloidogyne incognita nationallicence Arabidopsis thaliana nationallicence immunolabelling nationallicence JIM(s) nationallicence migration nationallicence Nobre M.J. Gravato Entomology and Nematology Department, IACR Rothamsted Harpenden, Herts AL52JQ, UK aut Mende N. von Entomology and Nematology Department, IACR Rothamsted Harpenden, Herts AL52JQ, UK aut Dolan L. Department of Cell Biology, John Innes Institute Colney Lane, Norwich NR4 7UH, UK aut Schmidt K.P. Instut für Phytopathologie, Universität Kiel Hermann-Rodewald-Strasse 9, D-2300 Kiel, Germany aut Evans K. Entomology and Nematology Department, IACR Rothamsted Harpenden, Herts AL52JQ, UK aut Mulligan B. Department of Life Sciences, University of Nottingham Nottingham NG72RD, UK aut Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1711-1720 0022-0957 46:11<1711 1995 46 exbotj https://doi.org/10.1093/jxb/46.11.1711 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.11.1711 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nobre M.J. Gravato Entomology and Nematology Department, IACR Rothamsted Harpenden, Herts AL52JQ, UK aut NATIONALLICENCE 700 1- Mende N. von Entomology and Nematology Department, IACR Rothamsted Harpenden, Herts AL52JQ, UK aut NATIONALLICENCE 700 1- Dolan L. Department of Cell Biology, John Innes Institute Colney Lane, Norwich NR4 7UH, UK aut NATIONALLICENCE 700 1- Schmidt K.P. Instut für Phytopathologie, Universität Kiel Hermann-Rodewald-Strasse 9, D-2300 Kiel, Germany aut NATIONALLICENCE 700 1- Evans K. Entomology and Nematology Department, IACR Rothamsted Harpenden, Herts AL52JQ, UK aut NATIONALLICENCE 700 1- Mulligan B. Department of Life Sciences, University of Nottingham Nottingham NG72RD, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1711-1720 0022-0957 46:11<1711 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford