caa a22 4500 397521197 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199511 xx s 000 0 eng 10.1093/jxb/46.11.1753 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.11.1753 Biosynthesis and partitioning of individual glucosinolates between pod walls and seeds and evidence for the occurrence of PAPS:desulphoglucosinolate sulphotransferase in seeds of oilseed rape (Brassica napus L.) [Elektronische Daten] [D. Toroser, H. Griffiths, C. Wood, D.R. Thomas] 35SO42−; and 35S-labelled glucosinolate precursors were administered to intact whole-pods and seeds to investigate the capacity of oilseed rape (Brassica napus L.) pod tissues to carry out reactions of the glucosinolate biosynthetic pathway. 35S-desulphobut-3-enyl and 35S-desulphoindol-3-ylmethyl glucosinolates were converted to their sulphonated ‘intact glucosinolate' homologues by isolated immature seeds. A neutral sulphur-containing fraction was isolated from pod walls and shown to be associated with glucosinolate biosynthesis. Further purification of this fraction showed the presence of desulphoglucosinolates, the penultimate intermediates in the glucosinolate biosynthetic pathway. Chemical characterization and quantification of these intermediates showed that their types and levels corresponded to the glucosinolate biosynthetic activity of pod-wall tissues. ‘Partition quotients' (Pq) were calculated for individual glucosinolates from 35S-labelling data and used to describe the apportionment of newly synthesized glucosinolates between pod walls and seeds. Results from continuous feeding studies with pods and 35SO42−; indicated that individual rapeseed glucosinolates have characteristic Pq values. © Oxford University Press RESEARCH PAPERS nationallicence biosynthesis nationallicence desulphoglucosinolates nationallicence glucosinolates nationallicence partitioning nationallicence rapeseed nationallicence Toroser D. Department of Biological and Nutritional Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut Griffiths H. Department of Agricultural and Environmental Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut Wood C. Department of Biological and Nutritional Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut Thomas D.R. Department of Biological and Nutritional Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1753-1760 0022-0957 46:11<1753 1995 46 exbotj https://doi.org/10.1093/jxb/46.11.1753 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.11.1753 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Toroser D. Department of Biological and Nutritional Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 700 1- Griffiths H. Department of Agricultural and Environmental Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 700 1- Wood C. Department of Biological and Nutritional Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 700 1- Thomas D.R. Department of Biological and Nutritional Sciences, The University of Newcastle upon Tyne Newcastle upon Tyne NE1 7RU, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1753-1760 0022-0957 46:11<1753 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford