caa a22 4500 397521227 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199511 xx s 000 0 eng 10.1093/jxb/46.11.1771 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.11.1771 Polyethylene glycol improves the in vitro growth of Brassica pollen tubes without loss in germination [Elektronische Daten] [K.R. Shivanna, V.K. Sawhney] The culture media for the in vitro pollen assay of Brassica species have so far shown good percentage germination, but limited pollen tube growth. It is reported here that by lowering sucrose concentration from 20% to 5 or 10 % and by adding polyethylene glycol 4000 in the culture medium, high percentage germination and high tube growth (10 times that in the standard media) can be achieved in three species of cultivated Brassica. This improved medium should be useful in fundamental and applied studies on the pollen biology of Brassica species. © Oxford University Press SHORT COMMUNICATIONS nationallicence Brassica nationallicence pollen nationallicence pollen germination nationallicence pollen tube growth nationallicence polyethylene glycol nationallicence Shivanna K.R. Department of Botany, University of Delhi Delhi 110007, India aut Sawhney V.K. Department of Biology, University of Saskatchewan 112 Science Place, Saskatoon, SK. S7N 5E2, Canada aut Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1771-1774 0022-0957 46:11<1771 1995 46 exbotj https://doi.org/10.1093/jxb/46.11.1771 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.11.1771 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shivanna K.R. Department of Botany, University of Delhi Delhi 110007, India aut NATIONALLICENCE 700 1- Sawhney V.K. Department of Biology, University of Saskatchewan 112 Science Place, Saskatoon, SK. S7N 5E2, Canada aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/11(1995-11), 1771-1774 0022-0957 46:11<1771 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford