caa a22 4500 397521308 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199502 xx s 000 0 eng 10.1093/jxb/46.2.249 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.2.249 Pinitol occurrence in soybean plants as affected by temperature and plant growth regulators [Elektronische Daten] [Changxin Guo, Derrick M. Oosterhuis] Unsuitable temperatures are frequently encountered by soybean (Glycine max L. Merr.) plants grown in the field. Certain polyols have been reported to protect plants from high temperature or frost damage. Controlled environment studies were conducted to investigate the effect of stressful temperature regimes on the content of pinitol (3-O-methyl-D-chiro-inositol) in soybean plants. Hydroponically-grown soybean plants were subjected to high (35/30 °C) or low (15/10 °C) day/night temperature stresses, and pinitol content in different plant parts was determined using high performance liquid chromatography (HPLC). A synthetic plant growth regulator, PGR-IV, was foliarly applied to the plants to evaluate its effect on pinitol content in different plant components. Uniformly-labelled 14C-glucose was fed into the leaves via the transpiration stream, and the effects of high temperature and EXP-S1089, another synthetic plant growth regulator, on the incorporation of 14C-glucose into pinitol was evaluated using HPLC separation and scintillation spectrometry. High-temperature stress significantly increased plant pinitol content and the incorporation of 14C-glucose into pinitol, but decreased the content of sucrose, glucose and fructose. Under low-temperature stress, there was hardly any change in pinitol content, but a drastic increase in soluble sugars. PGR-IV enhanced pinitol translocation from leaves to stems and roots, while EXP-S1089 increased pinitol/sucrose ratio. Accumulation of pinitol may be an adjustment mechanism of the plant to reduce high-temperature damage, but not low-temperature injuries. © Oxford University Press Research Papers nationallicence Pinitol nationallicence soybean nationallicence temperature nationallicence plant growth regulator nationallicence Guo Changxin Altheimer Laboratory, Department of Agronomy, University of Arkansas, Fayetteviile, AR 72703, USA aut Oosterhuis Derrick M. Altheimer Laboratory, Department of Agronomy, University of Arkansas, Fayetteviile, AR 72703, USA aut Journal of Experimental Botany Oxford University Press 46/2(1995-02), 249-253 0022-0957 46:2<249 1995 46 exbotj https://doi.org/10.1093/jxb/46.2.249 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.2.249 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Guo Changxin Altheimer Laboratory, Department of Agronomy, University of Arkansas, Fayetteviile, AR 72703, USA aut NATIONALLICENCE 700 1- Oosterhuis Derrick M. Altheimer Laboratory, Department of Agronomy, University of Arkansas, Fayetteviile, AR 72703, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/2(1995-02), 249-253 0022-0957 46:2<249 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford