caa a22 4500 397521324 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.9.1205 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.9.1205 The effect of auxin starvation on the growth of auxin-dependent tobacco cell culture: dynamics of auxin-binding activity and endogenous free IAA content [Elektronische Daten] [Eva Zažimalová, Zdeněk Opatrný, Alena Březinová, Josef Eder] The growth of a cell strain derived from the stem pith of tobacco (Nicotiana tabacum L., cv. Virginia Bright Italia) was investigated in subcultures grown at various levels of synthetic auxins. Both partial and complete auxin starvation resulted in a decrease of the frequency of cell division. For these treatments the endogenous free indole-3-acetic acid content increased substantially at the commencement of the exponential growth phase. The possibility that the receptivity of the cells to auxin changed during the growth cycle was examined by measuring the activity of a membrane-bound auxin-binding site. In subcultures grown in a medium with an optimal auxin concentration the maximum auxin-binding activity was restricted to the end of the exponential growth phase. In the cells cultivated in partially or completely auxin deprived media the auxin-binding activity increased to varying extents. These results probably reflect mechanisms controlling both the intracellular content of free auxin and the sensitivity of the cells to exogenous auxin supply (including auxin binding) with respect to the cell division and/or growth © Oxford University Press RESEARCH PAPERS nationallicence Nicotiana tabacum L nationallicence plant cell culture nationallicence IAA nationallicence auxin-binding site nationallicence cell division nationallicence Zažimalová Eva De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, The Academy of Sciences of the Czech Republic Ke dvoru 16/15, 166 30 Prague 6, Czech Republic aut Opatrný Zdeněk Research Institute of Crop Production Drnovská 507, 16100 Prague 6, Czech Republic aut Březinová Alena Institute of Experimental Botany, Academy of Sciences of the Czech Republic Ke dvoru 16/15, 16630 Prague 6, Czech Republic aut Eder Josef Institute of Experimental Botany, Academy of Sciences of the Czech Republic Ke dvoru 16/15, 16630 Prague 6, Czech Republic aut Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1205-1213 0022-0957 46:9<1205 1995 46 exbotj https://doi.org/10.1093/jxb/46.9.1205 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.9.1205 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zažimalová Eva De Montfort University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany, The Academy of Sciences of the Czech Republic Ke dvoru 16/15, 166 30 Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Opatrný Zdeněk Research Institute of Crop Production Drnovská 507, 16100 Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Březinová Alena Institute of Experimental Botany, Academy of Sciences of the Czech Republic Ke dvoru 16/15, 16630 Prague 6, Czech Republic aut NATIONALLICENCE 700 1- Eder Josef Institute of Experimental Botany, Academy of Sciences of the Czech Republic Ke dvoru 16/15, 16630 Prague 6, Czech Republic aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1205-1213 0022-0957 46:9<1205 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford