caa a22 4500 397521375 CHVBK 20180308164633.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.special_issue.1423 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.special_issue.1423 Effect of nitrate concentration during growth on carbon partitioning and sink strength in chicory [Elektronische Daten] [R. Ameéziane, M.A. Limami, G. Noctor, J.-F. Morot-Gaudry] Chicory plants (Cichorium intybus), which produce a tuberized root containing high concentrations of fructans, were grown with nitrate at either optimal (4 mol m−3) or suboptimal (0.6 mol m−3) concentration. At 120 DAS (days after sowing), shoot/root dry mass ratio in the plants grown at low N was less than one-third of that of plants grown at the higher concentration. This reflected differences in shoot dry mass, as root dry mass was similar in the two treatments. Pulse-chase experiments with 14CO2 showed increased allocation of carbon to the root and decreased partitioning to the growing shoot in the plants grown at low N. Root activities of three enzymes involved in sucrose and fructan metabolism were measured, in an attempt to identify the factors which determine sink strength. Whereas invertase activity showed little difference between the treatments, sucrose synthase and particularly sucrose-sucrose fructosyl transferase were higher in the roots of plants grown at low N. Activity of SST was also much greater in roots of young plants synthesizing large amounts of fructan than in mature roots no longer producing fructan. It is, therefore, suggested that SST plays a role in determining the sink strength. Possible interactions between N status and SST activity/sink strength are discussed. © 1995 Oxford University Press Environmental Interactions nationallicence Chicory nationallicence Cichorium intybus nationallicence nitrogen nationallicence sink strength nationallicence carbon partitioning nationallicence sucrose-sucrose fructosyl transferase nationallicence sucrose synthase nationallicence Ameéziane R. Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut Limami M.A. Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut Noctor G. Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut Morot-Gaudry J.-F Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1423-1428 0022-0957 46:special_issue<1423 1995 46 exbotj https://doi.org/10.1093/jxb/46.special_issue.1423 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.special_issue.1423 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ameéziane R. Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut NATIONALLICENCE 700 1- Limami M.A. Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut NATIONALLICENCE 700 1- Noctor G. Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut NATIONALLICENCE 700 1- Morot-Gaudry J.-F Laboratoire du Métabolisme, INRA, Route de Saint-Cyr, 78026 Versailles cedex, France aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1423-1428 0022-0957 46:special_issue<1423 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford