caa a22 4500 397521464 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.9.1131 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.9.1131 The nodulation of micro-propagated plants of Parasponia andersonii by tropical legume rhizobia [Elektronische Daten] [G. Webster, P. R. Poulton, E. C. Cocking, M. R. Davey] A simple clonal micro-propagation system for Parasponia andersonii was employed to study the nodulation response of this non-legume to inoculation by the broad host range Rhizobium sp. NGR234, isolated from Lablab purpureus, and also to tropical legume rhizobia isolated from Aeschynomene species. Partially effective nodules, assayed by acetylene reduction and 15N dilution procedures, were induced with strain NGR234 and its spontaneous streptomycinresistant mutant ANU240. Effective nodules were produced by one of the Aeschynomene strains (ORS302) tested, with rates of acetylene reduction comparable to those of root nodules produced by Bradyrhizobium strain CP279, originally isolated from P. andersonii. Light and transmission electron microscopy showed that there was a correlation between the nitrogen fixing capability of the symbiosis between NGR234 and Parasponia and the number of persistent infection (fixation) threads within the nodule cells. © Oxford University Press RESEARCH PAPERS nationallicence Parasponia nationallicence Bradyrhizobium nationallicence Rhizobium nationallicence Aeschynomene nationallicence micro-propagation nationallicence root nodules nationallicence nitrogen fixation nationallicence Webster G. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham Nottingham NG72RD, UK aut Poulton P. R. Soil Science Department, IACR Rothamsted Experimental Station Harpenden, Hertfordshire AL52JQ, UK aut Cocking E. C. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham Nottingham NG72RD, UK aut Davey M. R. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham Nottingham NG72RD, UK aut Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1131-1137 0022-0957 46:9<1131 1995 46 exbotj https://doi.org/10.1093/jxb/46.9.1131 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.9.1131 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Webster G. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham Nottingham NG72RD, UK aut NATIONALLICENCE 700 1- Poulton P. R. Soil Science Department, IACR Rothamsted Experimental Station Harpenden, Hertfordshire AL52JQ, UK aut NATIONALLICENCE 700 1- Cocking E. C. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham Nottingham NG72RD, UK aut NATIONALLICENCE 700 1- Davey M. R. Plant Genetic Manipulation Group, Department of Life Science, University of Nottingham Nottingham NG72RD, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/9(1995-09), 1131-1137 0022-0957 46:9<1131 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford