caa a22 4500 397521510 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199505 xx s 000 0 eng 10.1093/jxb/46.5.551 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.5.551 Possible involvement of ethylene-activated β-cyanoalanine synthase in the regulation of cocklebur seed germination [Elektronische Daten] [Ryo Hasegawa, Akiko Maruyama, Hiroshi Sasaki, Tomoko Tada, Yohji Esashi] A possible involvement of β-cyanoalanine synthase (CAS: EC 4.4.1.9) in germination processes of seeds was demonstrated using pre-soaked upper seeds of cocklebur (Xanthium pennsylvanicum Wallr.). Pretreatment in anoxia not only with KCN but also cysteine, as the substrates for CAS, stimulated the subsequent germination of cocklebur seeds in air. However, the effect of cysteine was manifested even in air when applied together with C2H4, and its effect was further enhanced in combination with KCN. The germination-stimulating effect of KCN was intensified by C2H4 only when 02 was present. In contrast, serine, another substrate of CAS, was effective in air only when combined with C2H4 and/or KCN. The addition of cysteine greatly reduced the cyanogenic glycoside content of seeds, but increased HCN evolution. On the other hand, glutathione did not have any effect on cocklebur seed germination, HCN evolution or bound cyanogen content, suggesting that cysteine is not acting as a reducing reagent. It is suggested that CAS regulates the process of cocklebur seed germination by the dual action of enlarging the pool of amino acids and supplying sulphydryl bases, the latter being more determinately important. Serine is effective only via the former action, while cysteine would act via both. © Oxford University Press Research Papers nationallicence Cyanide nationallicence cyanogenic glycoside nationallicence β-cyanoalanine synthase nationallicence seed germination nationallicence Xanthium pennsylvanicum nationallicence Hasegawa Ryo Agricultural Science Research Laboratory, Takarazuka Research Centre Sumitomo Chemical Co., Ltd 2-14-Chome, Takatsukasa, Takarazuka City, Hyogo 665, Japan aut Maruyama Akiko Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut Sasaki Hiroshi Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut Tada Tomoko Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut Esashi Yohji Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut Journal of Experimental Botany Oxford University Press 46/5(1995-05), 551-556 0022-0957 46:5<551 1995 46 exbotj https://doi.org/10.1093/jxb/46.5.551 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.5.551 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hasegawa Ryo Agricultural Science Research Laboratory, Takarazuka Research Centre Sumitomo Chemical Co., Ltd 2-14-Chome, Takatsukasa, Takarazuka City, Hyogo 665, Japan aut NATIONALLICENCE 700 1- Maruyama Akiko Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut NATIONALLICENCE 700 1- Sasaki Hiroshi Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut NATIONALLICENCE 700 1- Tada Tomoko Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut NATIONALLICENCE 700 1- Esashi Yohji Department of Biological Science, Tohoku University Kawauchi, Aobaku, Sendai 980, Japan aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/5(1995-05), 551-556 0022-0957 46:5<551 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford