caa a22 4500 397521529 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199503 xx s 000 0 eng 10.1093/jxb/46.3.337 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.3.337 Transformation of chicory and expression of the bacterial uidA and nptll genes in the transgenic regenerants [Elektronische Daten] [Malika Abid, Benoît Palms, Riet Derycke, Jean Pierre Tissier, Serge Rambour] Transgenic chicory plants were obtained from different explants co-cultured with Agrobacterium tumefaciens. Among tap-root, leaf and cotyledonary tissues, etiolated cotyledons showed the greatest competence for transformation. The Agrobacterium strains used contained either pGSGLUC1 or pTDE4 as a vector which carry both the neomycin phosphotransferase II gene (nptll) for kanamycin resistance and β-glucuronidase gene (uidA) under the control of different promoters. Transformation was confirmed by NPTII enzymatic assay, histochemical analysis of GUS activity and DNA hybridization. Transgenic plants expressed both marker genes in root and shoot tissues. In leaves, GUS activity was expressed in all tissue types, whatever the nature of the promoter. Nevertheless, variable heterogeneous patterns of expression were observed in the different root tissues. Differential expression of the GUS fusions controlled by the dual TR or the CaMV 35S promoters are discussed. © Oxford University Press Research Papers nationallicence Chicory nationallicence genetic transformation nationallicence GUS activity nationallicence kanamycin resistance nationallicence Abid Malika Physiologie et Génétique Moléculaire Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq cedex, France aut Palms Benoît Physiologie et Génétique Moléculaire Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq cedex, France aut Derycke Riet Laboratorium voor Genetica, Rijksuniversiteit Gent K.L. Ledeganckstraat 33, B-9000 Ghent, Belgium aut Tissier Jean Pierre Laboratoire Génie des Procédés et Technologies Alimentaires INRA, F-59651 Villeneuve d'Ascq cedex, France aut Rambour Serge Physiologie et Génétique Moléculaire Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq cedex, France aut Journal of Experimental Botany Oxford University Press 46/3(1995-03), 337-346 0022-0957 46:3<337 1995 46 exbotj https://doi.org/10.1093/jxb/46.3.337 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.3.337 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abid Malika Physiologie et Génétique Moléculaire Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq cedex, France aut NATIONALLICENCE 700 1- Palms Benoît Physiologie et Génétique Moléculaire Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq cedex, France aut NATIONALLICENCE 700 1- Derycke Riet Laboratorium voor Genetica, Rijksuniversiteit Gent K.L. Ledeganckstraat 33, B-9000 Ghent, Belgium aut NATIONALLICENCE 700 1- Tissier Jean Pierre Laboratoire Génie des Procédés et Technologies Alimentaires INRA, F-59651 Villeneuve d'Ascq cedex, France aut NATIONALLICENCE 700 1- Rambour Serge Physiologie et Génétique Moléculaire Végétales, Université des Sciences et Technologies de Lille F-59655 Villeneuve d'Ascq cedex, France aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/3(1995-03), 337-346 0022-0957 46:3<337 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford