caa a22 4500 397521545 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199510 xx s 000 0 eng 10.1093/jxb/46.10.1515 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.10.1515 The role of sucrose synthase in the response of soybean nodules to drought [Elektronische Daten] [E.M. González, A.J. Gordon, C.L. James, C. Arrese-lgor] Experiments were carried out to investigate the effects of drought stress on enzymatic activities related to carbon and nitrogen metabolism in soybean nodules. Gradual drought stress, imposed by withholding water nutrients, resulted in declines in the water potential of leaves and nodules consistent with a significant decline in N2 fixation. However, the amounts of nitrogenase components 1 and 2 were virtually unaffected by drought stress. Similarly, no significant changes could be detected in aspartate aminotransferase, phosphoenolpyruvate carboxylase, glutamine synthetase or alkaline invertase activities throughout the experiment. In contrast, sucrose synthase (SS), one of the enzymes involved in sucrose metabolism in legume nodules, declined dramatically in activity and in content within a few days of withholding water. Coincident with this decline in SS activity were significant increases in the nodule contents of sucrose, total free amino acids and ureides. The amounts of proline, however, did not increase until some days later. It is suggested that SS may play a key role in the regulation of nodule carbon metabolism and, therefore, of nitrogen fixation under drought stress conditions. © Oxford University Press Research Papers nationallicence Glycine max nationallicence soybean nationallicence nodule metabolism nationallicence drought stress nationallicence sucrose synthase nationallicence González E.M. Departamento de Ciencias del Medio Natural, Universidad Pública de Havana, Campus Arrosadia E-31006 Pamplona, Spain aut Gordon A.J. Department of Cell Biology, Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY23 3EB, UK aut James C.L. Department of Cell Biology, Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY23 3EB, UK aut Arrese-lgor C. Departamento de Ciencias del Medio Natural, Universidad Pública de Havana, Campus Arrosadia E-31006 Pamplona, Spain aut Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1515-1523 0022-0957 46:10<1515 1995 46 exbotj https://doi.org/10.1093/jxb/46.10.1515 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.10.1515 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- González E.M. Departamento de Ciencias del Medio Natural, Universidad Pública de Havana, Campus Arrosadia E-31006 Pamplona, Spain aut NATIONALLICENCE 700 1- Gordon A.J. Department of Cell Biology, Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY23 3EB, UK aut NATIONALLICENCE 700 1- James C.L. Department of Cell Biology, Institute of Grassland and Environmental Research, Plas Gogerddan Aberystwyth, Dyfed SY23 3EB, UK aut NATIONALLICENCE 700 1- Arrese-lgor C. Departamento de Ciencias del Medio Natural, Universidad Pública de Havana, Campus Arrosadia E-31006 Pamplona, Spain aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/10(1995-10), 1515-1523 0022-0957 46:10<1515 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford