caa a22 4500 397521588 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199505 xx s 000 0 eng 10.1093/jxb/46.5.489 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.5.489 Use of transfer function and compartmental analysis to quantify 11C-labelled photoassimilate export from wheat leaves [Elektronische Daten] [N. Keutgen, G.W. Roeb, P.E.H. Minchin, F. Führ] Compartmental analysis of tracer loss from a leaf after pulse-labelling with carbon isotopes has often been used to infer the flow of photosynthate through the leaf. Recently, a more general approach has been suggested based upon estimation of the transfer function using data from pulse-labelling as well as continuouslabelling experiments. A comparison of these two approaches shows that with the same data set they give equivalent physiological interpretations. The measured decline of 11C activity from a wheat leaf after 11CO2 pulse-labelling was extrapolated by compartmental as well as transfer function analysis. Both methods estimated a 66.4% loss of the initially fixed 11C due to export and respiration. The advantage of transfer function analysis, however, is its applicability to continuous-labelling experiments. The model allows the use of the net photosynthetic rate as the reference (100%) value. Data from continuous-labelling experiments with wheat plants indicate diurnal variations in the export of freshly labelled assimilate of between 32.7% and 43.6% of net photosynthesis. © Oxford University Press Research Papers nationallicence Triticum aestivum L nationallicence 11CO2 nationallicence carbon partitioning nationallicence transfer function analysis nationallicence compartmental analysis nationallicence Keutgen N. Institute of Radioagronomy, KFA Research Centre Jülich GmbH D-52425 Jülich, Germany aut Roeb G.W. Institute of Radioagronomy, KFA Research Centre Jülich GmbH D-52425 Jülich, Germany aut Minchin P.E.H. Horticultural and Food Research Institute of New Zealand Ltd PO Box 31-313, Lower Hutt, New Zealand aut Führ F. Institute of Radioagronomy, KFA Research Centre Jülich GmbH D-52425 Jülich, Germany aut Journal of Experimental Botany Oxford University Press 46/5(1995-05), 489-496 0022-0957 46:5<489 1995 46 exbotj https://doi.org/10.1093/jxb/46.5.489 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.5.489 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Keutgen N. Institute of Radioagronomy, KFA Research Centre Jülich GmbH D-52425 Jülich, Germany aut NATIONALLICENCE 700 1- Roeb G.W. Institute of Radioagronomy, KFA Research Centre Jülich GmbH D-52425 Jülich, Germany aut NATIONALLICENCE 700 1- Minchin P.E.H. Horticultural and Food Research Institute of New Zealand Ltd PO Box 31-313, Lower Hutt, New Zealand aut NATIONALLICENCE 700 1- Führ F. Institute of Radioagronomy, KFA Research Centre Jülich GmbH D-52425 Jülich, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/5(1995-05), 489-496 0022-0957 46:5<489 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford