caa a22 4500 397521626 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/jxb/46.6.617 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.6.617 Metabolic changes in soybean nodules after inhibition of nitrogen fixation by treatment with oxygen [Elektronische Daten] [Norio Suganuma, Thomas A. LaRue] Metabolites that accumulated in soybean [Glycine max (L.) Merr.] nodules after inhibition of nitrogen fixation were analysed to determine what carbon compounds the bacteroids might obtain from their host. Exposure of roots of intact soybean plants to 100% O2 for 5 min caused a decrease in acetylene reduction activity within 10 min and then the activity recovered only slowly. Analysis of carbohydrates, organic acids, volatile compounds and amino acids in extracts of nodules revealed that succinate, malate and alanine all accumulated within 10 min after treatment with O2. The concentrations of sucrose, acetone, tyrosine, valine, isoleucine, leucine, and ornithine in the nodules increased slowly after such treatment. The results are discussed in terms of carbon sources for supporting nitrogen fixation of soybean bacteroids. © Oxford University Press Research Papers nationallicence Glycine max nationallicence carbon metabolism nationallicence nitrogen fixation nationallicence nodules nationallicence Suganuma Norio Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, USA aut LaRue Thomas A. Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, USA aut Journal of Experimental Botany Oxford University Press 46/6(1995-06), 617-622 0022-0957 46:6<617 1995 46 exbotj https://doi.org/10.1093/jxb/46.6.617 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.6.617 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Suganuma Norio Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, USA aut NATIONALLICENCE 700 1- LaRue Thomas A. Boyce Thompson Institute for Plant Research, Cornell University, Tower Road, Ithaca, NY 14853, USA aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/6(1995-06), 617-622 0022-0957 46:6<617 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford