caa a22 4500 397521693 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/jxb/46.6.675 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.6.675 Temporal and nutritional factors modulate responses to abscisic acid and osmoticum in their regulation of storage protein synthesis in developing seeds of alfalfa (Medicago sativa L.) [Elektronische Daten] [Nanfei Xu, J. Derek Bewley] Storage protein synthesis during alfalfa (Medicago sativa L.) seed development displays a temporal pattern and is regulated both by ABA and low osmotic potentials. Deposition of the 2S, 7S and 11S storage proteins occurs maximally at the mid- to late stages of development. Osmoticum, but not ABA alone could effectively induce storage protein synthesis at early stages of development in isolated alfalfa embryos placed on water for several days. Neither ABA nor osmoticum alone could maintain storage protein synthesis in isolated late-stage embryos placed on water. On the other hand, isolated developing embryos cultured on a nutrient (Murashige and Skoog) medium could maintain storage protein synthesis in the presence of ABA and osmoticum, alone or in combination, for up to 14 d after being dissected from the pod. Of the components of this medium, the inorganic salts appeared to be the most important. The response to ABA and osmoticum varied with the time of isolation during development with the greatest enhancement of storage protein synthesis in isolated embryos coinciding with the time of maximum synthesis within the seed when in planta. Addition of ABA and osmoticum after the time of maximum storage protein synthesis did not elevate the amount of synthesis, but rather prolonged the time over which it could take place. Thus, while the amplitude of storage protein synthesis could be modified by ABA or osmoticum, the inherent temporal pattern, with maximum synthesis occurring only at mid- to late stages of embryo development, could not. © Oxford University Press Research Papers nationallicence Embryogenesis nationallicence Medicago sativa L nationallicence alfalfa nationallicence abscisic acid nationallicence osmotic potential nationallicence storage protein synthesis nationallicence nutrient supply nationallicence Xu Nanfei Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada aut Bewley J. Derek Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada aut Journal of Experimental Botany Oxford University Press 46/6(1995-06), 675-686 0022-0957 46:6<675 1995 46 exbotj https://doi.org/10.1093/jxb/46.6.675 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.6.675 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Nanfei Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada aut NATIONALLICENCE 700 1- Bewley J. Derek Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/6(1995-06), 675-686 0022-0957 46:6<675 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford