caa a22 4500 397521715 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199512 xx s 000 0 eng 10.1093/jxb/46.12.1895 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.12.1895 Histone H1 during germination of pea seeds: an analysis by electrophoretic and immunofluorimetric methods [Elektronische Daten] [Walter Dicorato, Christian Savini, Marcella Bracale, Sergio Sgorbati, Maria Grazia Galli] Histone H1 has sometimes been reported to undergo quantitative and/or qualitative changes during germination of seeds, but the overall picture on this topic is far from clear. A re-examination of this problem seemed therefore of interest. The H1 content from root tips of ungerminated pea embryos (with nuclei in a completely quiescent state) have been compared with that of seedlings germinated for 48 and 96 h (with nuclei actively involved in transcription and replication). As well as conventional analysis by gel electrophoresis, which seemed to indicate a striking selective decrease of H1 content as germination proceeded, a completely different approach was used: biparametric analysis with flow cytometry, utilizing the indirect immunofluorescence of nuclei previously treated with a monospecific polyclonal antibody raised against pea H1. This proved to be a powerful approach and the results ruled out the possibility of a true depletion of H1 during germination events. Data from SDS electrophoresis of whole nuclear proteins and Northern blot analysis of the H1b cDNA during maturation and germination of pea seeds supported this conclusion. © Oxford University Press Research Papers nationallicence Chromatin nationallicence cytofluorimetry nationallicence germination nationallicence histone H1 nationallicence Pisum sativum nationallicence Dicorato Walter CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut Savini Christian CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut Bracale Marcella CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut Sgorbati Sergio CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut Galli Maria Grazia CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut Journal of Experimental Botany Oxford University Press 46/12(1995-12), 1895-1803 0022-0957 46:12<1895 1995 46 exbotj https://doi.org/10.1093/jxb/46.12.1895 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.12.1895 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dicorato Walter CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut NATIONALLICENCE 700 1- Savini Christian CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut NATIONALLICENCE 700 1- Bracale Marcella CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut NATIONALLICENCE 700 1- Sgorbati Sergio CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut NATIONALLICENCE 700 1- Galli Maria Grazia CNR and Dipartimento di Biologia, Sezione Botanica Generale, Universita' degli Studi di Milano, via Celoria 26, 1-20133 Milano, Italy aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/12(1995-12), 1895-1803 0022-0957 46:12<1895 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford