caa a22 4500 397521782 CHVBK 20180308164634.0 cr unu---uuuuu 161202e199504 xx s 000 0 eng 10.1093/jxb/46.4.401 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.4.401 Measurement of the rate of CO2 solubilization in leaf tissue 4 [Elektronische Daten] [V. Oja, A. Laisk] A novel method for the measurement of the rate of CO2 exchange caused by solubilization and bicarbonate formation in leaves is presented. The method is based on the measurement of changes in O2 concentration, caused by the uptake or evolution of CO2 from the gas flow as a result of solubilization in or desolubilization from leaf tissue, by means of a zirconium-oxide O2 analyser. The advantages of the new method are a fast response and a lack of interference by the CO2 contained in the leaf chamber and leaf intercellular spaces. The method can be used for the investigation of the kinetics of CO2 transport from leaf intercellular spaces to chloroplasts and the role of carbonic anhydrase in this transport and the investigation of mechanistic and ecological aspects of buffering, proton translocation and other processes controlling pH in chloroplasts of intact leaves. © Oxford University Press Articles nationallicence Leaf nationallicence carbon dioxide nationallicence pH nationallicence Oja V. Institute of Molecular and Cell Biology, Tartu University, Riia 181, EE2400 Tartu, Estonia aut Laisk A. Institute of Molecular and Cell Biology, Tartu University, Riia 181, EE2400 Tartu, Estonia aut Journal of Experimental Botany Oxford University Press 46/4(1995-04), 401-406 0022-0957 46:4<401 1995 46 exbotj https://doi.org/10.1093/jxb/46.4.401 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.4.401 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Oja V. Institute of Molecular and Cell Biology, Tartu University, Riia 181, EE2400 Tartu, Estonia aut NATIONALLICENCE 700 1- Laisk A. Institute of Molecular and Cell Biology, Tartu University, Riia 181, EE2400 Tartu, Estonia aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/4(1995-04), 401-406 0022-0957 46:4<401 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford