caa a22 4500 397521839 CHVBK 20180308164635.0 cr unu---uuuuu 161202e199504 xx s 000 0 eng 10.1093/jxb/46.4.415 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.4.415 A mutation at the rb gene, lowering ADPGPPase activity, affects storage product metabolism of pea seed coats [Elektronische Daten] [Christine Rochat, Sylvie Wuillème, Jean-Pierre Boutin, Cliff L. Hedley] Seed coat development was studied on two nearisogenic lines of peas (Pisum sativum L.): RbRb (wild type, round seed) and rbrb (wrinkled seed). A mutation at the rb locus modifies the dry seed shape and reduces the starch content of the embryo. This mutation is now known to affect the activity of ADPGlucose pyrophosphorylase, a key enzyme in the starch biosynthetic pathway. We have investigated the effects of the rb mutation on seed coat development and found that the mutation reduces the growth rate and starch content in this organ. However, experiments on the kinetics of 14C-sucrose loading showed that starch synthesis from unloaded sucrose occurred in the seed coat for both mutant and wild-type lines. In addition, the sucrose concentration was increased and amino acid concentration decreased such that the nutritional balance of the embryos was affected. However, osmolality of the seed coat cells was not affected, suggesting a regulatory process which allows the maintenance of the import of assimilates in the seeds of either line. © Oxford University Press Articles nationallicence ADPGlucose pyrophosphorylase nationallicence seed coat nationallicence seed development nationallicence starch metabolism nationallicence wrinkled seed nationallicence Rochat Christine Laboratoire du Mètabolisme, INRA, Route de St Cyr F-78026 Versailles cedex, France aut Wuillème Sylvie Laboratoire du Mètabolisme, INRA, Route de St Cyr F-78026 Versailles cedex, France aut Boutin Jean-Pierre Laboratoire du Mètabolisme, INRA, Route de St Cyr F-78026 Versailles cedex, France aut Hedley Cliff L. Department of Applied Genetics, John Innes Institute Colney Lane, Norwich NR4 7UH, UK aut Journal of Experimental Botany Oxford University Press 46/4(1995-04), 415-421 0022-0957 46:4<415 1995 46 exbotj https://doi.org/10.1093/jxb/46.4.415 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.4.415 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Rochat Christine Laboratoire du Mètabolisme, INRA, Route de St Cyr F-78026 Versailles cedex, France aut NATIONALLICENCE 700 1- Wuillème Sylvie Laboratoire du Mètabolisme, INRA, Route de St Cyr F-78026 Versailles cedex, France aut NATIONALLICENCE 700 1- Boutin Jean-Pierre Laboratoire du Mètabolisme, INRA, Route de St Cyr F-78026 Versailles cedex, France aut NATIONALLICENCE 700 1- Hedley Cliff L. Department of Applied Genetics, John Innes Institute Colney Lane, Norwich NR4 7UH, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/4(1995-04), 415-421 0022-0957 46:4<415 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford