caa a22 4500 39752188X CHVBK 20180308164635.0 cr unu---uuuuu 161202e199509 xx s 000 0 eng 10.1093/jxb/46.special_issue.1377 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.special_issue.1377 Functioning of the tonoplast in vacuolar C-storage and remobilization in crassulacean acid metabolism [Elektronische Daten] [Ulrich Lüttge, Elke Fischer-Schliebs, Rafael Ratajczak, Detlef Kramer, Ekkehart Berndt, Manfred Kluge] Nocturnal fixation of CO2 and storage of carbon in the form of malic acid in vacuoles of photosynthetically active cells are key processes of crassulacean acid metabolism (CAM). A prerequisite for malate transport into the vacuole is the concerted action of primary-active H+ -pumps, i.e. the tonoplast V0V1-H+-ATPase and H+ -PPiase, and a secondary-active carboxylate transporter. During the transition from C3-photosynthesis to CAM the H+ -ATPase of the C3/CAM-inter-mediate plant Mesembryanthemum crystallinum undergoes particular structural changes indicating an adaptation of the enzyme to the requirements for an increased capacity of tonoplast transport systems. The role of the H+-PPiase in energization of carbon transport in CAM remains unclear. While in Kalanchoë blossfeldiana cv. Tom Thumb, where CAM is induced by shortening of daylength, there is a higher expression of the H+-PPiase in the CAM state as compared to the C3-state, the enzyme disappears in M. crystallinum when CAM is induced by salt stress. However, the H+-PPiase could also be involved in the regulation of the H+-ATPase as shown by the PPistimulated ATP-dependent H+-transport activity found in K. daigremontiana and K. blossfeldiana cv. Tom Thumb. Although the tonoplast carboxylate transporter has not yet been identified, the kinetic parameters of the enzyme were characterized after partial purification and functional reconstitution into proteo-liposomes. While the influx of malate into the vacuole is mediated by the co-operation of different enzymes, the regulation of the passive efflux of malic acid from the tonoplast during the day may depend on biophysical membrane properties, i.e. the degree of order of the vacuolar membrane, which seems to be modulated by Ca2+. © 1995 Oxford University Press Photorespiration: Alternative Pathways: Carbon Allocation nationallicence Crassulacean acid metabolism nationallicence carbon storage nationallicence carboxylate transporter nationallicence tonoplast nationallicence H+ -PPiase nationallicence V0V1-H+ - ATPase nationallicence Lüttge Ulrich Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut Fischer-Schliebs Elke Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut Ratajczak Rafael Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut Kramer Detlef Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut Berndt Ekkehart Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut Kluge Manfred Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1377-1388 0022-0957 46:special_issue<1377 1995 46 exbotj https://doi.org/10.1093/jxb/46.special_issue.1377 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.special_issue.1377 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lüttge Ulrich Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Fischer-Schliebs Elke Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Ratajczak Rafael Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Kramer Detlef Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Berndt Ekkehart Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 700 1- Kluge Manfred Institut für Botanik, Technische Hochschule Darmstadt, Schnittspahnstraße 3-5, D-64287 Darmstadt, Germany aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/special_issue(1995-09), 1377-1388 0022-0957 46:special_issue<1377 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford