caa a22 4500 397522037 CHVBK 20180308164635.0 cr unu---uuuuu 161202e199503 xx s 000 0 eng 10.1093/jxb/46.3.277 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.3.277 Changes in patterns of protein synthesis during haustorial development of Striga hermonthica (Del.) Benth. seedlings [Elektronische Daten] [Adriana Stranger, Alex Murphy, Joe M. Corbett, Mike J. Dunn, G. Paul Bolwell, George R. Stewart] This study focuses upon the developmental transition of the parasitic plant Striga hermonthica from its freeliving state (germinated seedling) to its parasitic state after development of an infection organ: the haustorium. A new method has been developed that allows the production of gram quantities of germinated and haustorially-induced Striga seedlings, thereby facilitating biochemical and molecular analysis of haustorial induction. Water-soluble proteins have been extracted from germinated seeds (stage A) and seedlings treated with 2,6-dimethoxy-p-benzoquinone (2,6-DMBQ) to induce haustorium (stage B). Samples were analysed by two-dimensional polyacrylamide gel electrophoresis and quantitative as well as qualitative differences could be observed. In particular a group of four highly abundant acidic proteins (molecular weight 39 kDa, pl 5.1, 5.3, 5.3, 5.6) and three other proteins (molecular weight 12 kDa, pl 6.9; 17 kDa, pl 4.4; 17 kDa, pl 4.45) were seen in stage A while at least four proteins (molecular weight 21.5 kDa, pl 6.4; 21.5 kDa, pl 6.3; 31 kDa, pl 5.1; 34 kDa, pl 6.2) were present in greater abundance in stage B. In order to compare watersoluble protein with newly synthesized protein patterns, mRNAs from the two stages of development were isolated and cell-free translation products analysed by 2-D PAGE. Two-D gels of cell-free translation products showed the appearance of six proteins in stage B (molecular weight ranging from 10 to 35 kDa) and the presence of three acidic proteins in stage A with one protein (molecular weight 40 kDa) very similar in size to the triplet of proteins in the water-soluble protein 2-D gels. © Oxford University Press Research Papers nationallicence Striga hermonthica (Del.) Benth nationallicence haustorium nationallicence 2-D PAGE nationallicence 2,6-DMBQ nationallicence translation in vitro nationallicence Stranger Adriana Department of Biology, University College London Darwin Building, Gower Street, London WC1E 6BT, UK aut Murphy Alex Department of Biology, University College London Darwin Building, Gower Street, London WC1E 6BT, UK aut Corbett Joe M. Heart Science Centre, National Heart and Lung Institute, Harefield Hospital Harefield, Middlesex UB9 6JH, UK aut Dunn Mike J. Heart Science Centre, National Heart and Lung Institute, Harefield Hospital Harefield, Middlesex UB9 6JH, UK aut Bolwell G. Paul Department of Biochemistry, Royal Holloway University of London Egham, Surrey TW20 OEX, UK aut Stewart George R. Department of Biology, University College London Darwin Building, Gower Street, London WC1E 6BT, UK aut Journal of Experimental Botany Oxford University Press 46/3(1995-03), 277-283 0022-0957 46:3<277 1995 46 exbotj https://doi.org/10.1093/jxb/46.3.277 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.3.277 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Stranger Adriana Department of Biology, University College London Darwin Building, Gower Street, London WC1E 6BT, UK aut NATIONALLICENCE 700 1- Murphy Alex Department of Biology, University College London Darwin Building, Gower Street, London WC1E 6BT, UK aut NATIONALLICENCE 700 1- Corbett Joe M. Heart Science Centre, National Heart and Lung Institute, Harefield Hospital Harefield, Middlesex UB9 6JH, UK aut NATIONALLICENCE 700 1- Dunn Mike J. Heart Science Centre, National Heart and Lung Institute, Harefield Hospital Harefield, Middlesex UB9 6JH, UK aut NATIONALLICENCE 700 1- Bolwell G. Paul Department of Biochemistry, Royal Holloway University of London Egham, Surrey TW20 OEX, UK aut NATIONALLICENCE 700 1- Stewart George R. Department of Biology, University College London Darwin Building, Gower Street, London WC1E 6BT, UK aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/3(1995-03), 277-283 0022-0957 46:3<277 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford