caa a22 4500 397522088 CHVBK 20180308164635.0 cr unu---uuuuu 161202e199506 xx s 000 0 eng 10.1093/jxb/46.6.655 doi (NATIONALLICENCE)oxford-10.1093/jxb/46.6.655 Enhancement of short- and medium-term expression of transgenes in embryogenic suspensions of Picea abies (L.) Karst [Elektronische Daten] [David Clapham, Gary Manders, Haile S. Yibrah, Sara von Arnold] Biolistic transfer and subsequent reporter gene expression was enhanced 3-20-fold in embryogenic suspension cultures of Picea abies by treatment with osmotica before and after bombardment. Myoinositol was more effective than mannitol + sorbitol and about as effective as sucrose in enhancing expression 1 d after bombardment. For both myoinositol and sucrose the optimal concentration was 0.25 M. Abscisic acid interacted with myoinositol to give a further enhancement. Maintaining the cells on 0.25 M myoinositol after bombardment enhanced the expression measured 10 d later. These results provide evidence for a metabolic component of the mechanism of osmotic enhancement in addition to an effect depending on partial plasmolysis at the time of bombardment. Expression driven by the polyubiquitin promoter 10 d after bombardment was more than twice that driven by an enhanced 35S, and 20 times that driven by a simple 35S promoter. Experiments using plasmids with and without scaffold attachment regions indicated that a greater proportion of the cells that expressed the reporter gene at 6 d compared with 1 d after bombardment had been integratively transformed. © Oxford University Press Research Papers nationallicence Abscisic acid nationallicence biolistics nationallicence osmotic enhancement nationallicence polyubiquitin promoter nationallicence scaffold attachment regions nationallicence Clapham David Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut Manders Gary Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut Yibrah Haile S. Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut von Arnold Sara Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut Journal of Experimental Botany Oxford University Press 46/6(1995-06), 655-662 0022-0957 46:6<655 1995 46 exbotj https://doi.org/10.1093/jxb/46.6.655 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/jxb/46.6.655 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clapham David Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut NATIONALLICENCE 700 1- Manders Gary Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut NATIONALLICENCE 700 1- Yibrah Haile S. Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut NATIONALLICENCE 700 1- von Arnold Sara Department of Forest Genetics, Swedish University of Agricultural Sciences, PO Box 7027, S-75007 Uppsala, Sweden aut NATIONALLICENCE 773 0- Journal of Experimental Botany Oxford University Press 46/6(1995-06), 655-662 0022-0957 46:6<655 1995 46 exbotj Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford